RNA to protein Flashcards
1
Q
What are 5 characteristics of the codon?
A
- 3 bases= one codon= one amino acid
- The code is non overlapping
- Degenerate code
- Start codon= Aug= Met
- Stop codon- UAA, UAG, UGA
2
Q
How is wobble base pairing achieved?
A
Deamination of adenine to create an inosine
Inosine can pair to U,C or A
3
Q
What happens to an amino acid before it forms an aminoacyl-tRNA
A
A synthetase primes the amino acid by adding AMP to the C terminus
4
Q
What does peptidyl transferase do?
A
Catalyses the amino acid addition to the growing protein strand
5
Q
What does EF1 do?
A
Causes a delay before the peptidyl transferase can act
- GTP hydrolysed to GDP
- EF1 dissociates from the tRNA
6
Q
What happens at the start of translation?
A
tRNA carrying Met with EIF2 binds to small subunit
7
Q
What binds to mRNA that still has a cap and a tail?
A
EIF-4G and EIF-4E
8
Q
Structural components of HS60?
A
Hydrophobic entrance
GroES cap
9
Q
What are amyloid plaques seen in?
A
Alzheimers- cross beta filaments
