Control of gene expression

Question 1

What charge do DNA binding proteins have?

Answer 1

Positive charge

Question 2

What does the ROX1 protein bind to?

Answer 2

HEM13 (3 sites)
ANB1 (4 sites)
ROX1 (1 site)

Question 3

What is the structure of a helix-turn-helix domain?

Answer 3

2 alpha helices joined together by an amino acid strand

Question 4

2 ways of identifying DNA binding proteins

Answer 4

1. DNA footprint

2. EMSA

Question 5

What are the types of transcription factor?

Answer 5

1. General T.factor

2. Specific/ regulatory

Question 6

How do regulatory transcription factors work?

Answer 6

1. Interact with the RNA polymerase complex
2. Alter acetylation of DNA
3. Bind to other transcription factor

Question 7

What do insulators do?

Answer 7

Block regulatory sequences from affecting neighbouring genes

Question 8

What does a low level of tyrptophan do?

Answer 8

Genetic switch is inactivated- gene is turned on

Question 9

3 ways to activate a transcription factor?

Answer 9

1. Activate ligand binding
2. Remove an inhibitory protein
3. Phosphorylation

Question 10

What needs to happen for the activation of the Ga1 gene?

Answer 10

Ga14 proteins (its product) to bind to upstream activating sequences