Recombinant technology Flashcards
1
Q
What stains DNA fragments?
A
Ethidium bromide
2
Q
What are the 2 types of DNA library?
A
- Genomic
2. cDNA
3
Q
What do cDNA libraries often sequence?
A
Short-subsequences of a cDNA sequence=
Expressed Sequence Tags
4
Q
What are dideoxynucleotides?
A
Similiar to deoxyribose nucleotides- but NO OH GROUP
Once bound to DNA the strand cannot be extended
terminates DNA synthesis
5
Q
What do we use in genomic sequencing?
A
Bacterial Artificial Chromosomes
6
Q
What is shotgun sequencing?
A
Many fragments of DNA- obtain reads from sanger method- several rounds of fragmentation and sequencing- assemble the overlapping reads into a continuous sequence
