Post transcriptional regulation

Question 1

Which genes are involved in drosophila male and female differentiation?

Answer 1

Sxl
Tra
Dsx

Question 2

What 2 antibodies can a B lymphocyte produce?

Answer 2

1. Long transcript- membrane bound antibody

2. Short transcript- secreted antibody

Question 3

What is the optimal start sequence in mRNA called?

Answer 3

Kozak sequence

Question 4

What is leaky scanning?

Answer 4

When the small ribosome can skip past the first AUG, stop at the 2nd or 3rd

Question 5

What are UTRS?

Answer 5

Untranslated region- of mRNA

Often house translational control elements

Question 6

What does ferritin do?

Answer 6

Stores iron in the cell (in the protein)

Question 7

What does transferrin do?

Answer 7

Transferrin is a receptor that imports iron into the cell

Question 8

What is eif-2b required for?

Answer 8

Required for the dissociation of GDP from eif-2

eif-2/GTP binds to Met tRNA to start ribosome scanning

Question 9

What is IRES?

Answer 9

IRES allows more than one gene to be present on an mRNA

They are stem loops in the mRNA- initiate formation of the ribosome independent of the cap/PolyA initiation complex

Question 10

What is required for IRES based initiation?

Answer 10

eif-4G