Rickettsia, Chlamydia Flashcards
Rickettsia and Chlamydia are gram __ bacteria
(-)
Rickettsia are transmitted by:
arthropod vectors (like mice)
What are the symptoms of Rickettsia?
local damage to blood v.v., inflammation (vasculitis), extravasated RBC’s
R. Rickettsia, after engulfment:
degrades the phagosome (with phospholipase A)
R. Coxiella, after engulfment:
remains in phagolysosome (likes acidic environment)
R. Ehrlichia, after engulfment:
remains in vacuole (phagosome), like TB
*doesn’t fuse with lysosome
Rocky Mountain Spotted Fever is a __-borne infection.
tick
For intracellular diseases, what drugs do we use?
Tetracycline and Chloramphenicol
What are the diagnosis methods for Rocky Mountain Spotted Fever?
MIF, DIF, and PCR
first two are antibody tests
Weil-Felix Test with Protease OX19 is associated with:
R. Rickettsia (it was a form of identifying the disease using WW2) *clumping = +
Number 1 bacterial STD?
Chlamydia
Number 1 microbial STD?
HPV
Rickettsia has tropism for:
vascular endothelial cells
Chlamydia has tropism for:
columnar epithelial cells lining mucous membranes
Chlamydia is ___, which means it has two forms.
dimorphic
Both Rickettsia and Chlamydia are obligate:
intracellular parasites
Growth of Chlamydia is inhibited by what drugs?
Tetracylines and Erythromycin
Quinolones
The infectious particle of Chlamydia is called the:
elementary body
The important particle in Chlamydia for reproduction is called:
reticular body
How does chlamydia avoid being killed by enzymes and lysosomes?
Because the phagocytic vesicle inhibits fusion with the lysosome
* Chlamydia can be killed if they are coated with antibodies; if they are, then the vesicle fuses with the lysosome so the lysosomal enzymes kill it
Discuss the growth cycle of C. trachomatis.
Elementary body is engulfed by columnar epithelial cell. Then, the elementary body divides a bunch and becomes reticulate bodies. The vesicle containing the EB and RB’s exit the cell via reverse endocytosis and go on to infect other cells.
Extracellular survival is a function of what form of chlamydia?
Elementary body (infectious particle)
What allows us to see inclusion bodies?
Iodine
What are the two types of antigens for Chlamydia?
Group and Specific antigens
Discuss group vs specific antigens.
Group = shared by all (heat stable, lipopolysaccharides) Specific = outer membrane proteins
List some general things about “Clam”ydia.
Trick: CLAMS have two shells!
- Chlamydia has two antibody types (group & specific)
- Chlamydia is dimorphic (two forms - reticular body and elementary body)
- We have two eyes - Chlamydia can cause eye problem s (specifically Trachoma)
One of the leading causes of blindness is caused by Chlamydia - what is it?
Ocular Trachomatis
Serovars A, B, Ba, and C infect:
conjunctiva (primarily)
Serovars D-K infect:
urogenital tract (like the DicK)
Serovars L1, L2, L2a, L3 infect:
Inguinal lymph nodes (can cause LGV)
Ancient eye disease?
Trachoma
50% of GC patients are co-infected with:
Chlamydia Trachomatis
What is lymphogranuloma venereum (LGV)?
- STD characterized by suppurative inguinal adenitis
- Regional lymph nodes are enlarged and painful
- Can lead to elephantiasis of penis or vulva
Treatment for LGV?
Cipro- (quinolones), Macrolides, Tetra-, Sulfonamides, Erythromycin
