Retics Lab Flashcards
Young form of rbc that mature
Reticulocyte
Retics mature for how many days
2-4 days
It has a reticulum network which are rna and protoporphyrin remnants
Retics
Nv of retics in adults
0.5-1.5%, 20,000-60,000 mm3
Nv in newborn
2.5-6.5%
Physiologic increase of retics
Among new born
Menstruating women
Among oreg women
Abnormal increase retics
Pt with HA
PB poisoning
Pt with leukemia
Pt with malaria
Abnormally dec retics
Idiopathic aplastic anemia brought a dec retics
Acute benzol poisoning
Suppression of retics
Acute benzol poisoning
Under wet method
New mb
Cook, meyer, tureen method
Seiverd method
In wet method what are under BCB
Seiverds method
Cook, meyer, tureen mtd
Primary used in wet method
New methylene blue method
Dry method
Schilling rapid method
Osgood wilhery
Sabin method
Sabin method using
Janus green and neutral red
Osgood wilhelry using
Methylene blue
Schilling rapid method using
Brilliant cresyl blue
Nv of retics
20-60X10,4 mm3
ARC NV
24-84X10,9/L
Follows the loaw of osmosis
Erythrocyte osmotic fragility test
Checking the stability of rbc
Eoft
Isotonic solution
0.85-0.9% nacl
Hypertonic soln
> 0.85% nacl
Hypotonic soln
Shrink
Hypertonic
Swell
Hypotonic
No changes in the morphology rbc
Isotonic
2 types of cell in EOFT
Sickle cell
Spherocyte
Fragile cell
Spherocyte
Resistant cell presence of HbS
Sickle cell
It test the stab of rbc ynder diff conc of hypotonic Nacl soln
Sanford method
Solution of sanford
0.50% nacl
Sanform preparation
12 test tube numbered witg 25-14
Specimen used in sanford
Fresh heparinuzed blood
Initial hemolysis normally in tube
16 or 17
Increase EOFT normally in tube
24
Complete eoft
19
Dec eoft initially
Tube 19
Tube with compact sediment and clear supernate
No hemolysis
1st from left that shows no sediments and with dark red supernate
Compelete hemolysis
1st tube from left not so compact sediment wupith pink supernate
Initial hemolysis
Dec eoft complete
15
Method under eoft
Sanford mtd Modified sanford Giffin and sanford Dacies mtd Fragiliograph mtd
Automated method of eoft
Fragiliograph mtd
Blood in diluted in hypotonic nacl soln buffer 7.4 oh added with po4
Dacies metd
Inc ofi
Hereditary spherocytosis
Dec ofi
Sickle cell
SIDA
Thalassemia
Jaundice
In caise of hemolytic anemia
Hereditary soberocytosis
Refers to the number of eosino per cubic mm of blood
Esinophil ct
Nv of eosinophil
150-300mm3
Eosinophil detects
Parasitic, allergicm normal adrenal fxn
Mtd under eosinophil
Pilot method
Friedman method
Randolph mtd
A sample of blood is diluted with a solution that selectively stain eo and eliminates leukocytes from vie
Randolph
Rgts under eosinophil
Propylene glycol Dstilled water Naco3 Philoxine Philoxine
Diluting agent of eosino
Distilled water
Sed to hemolyse rna
Propylene glycol
Make other cell invisible and enhances eosinophil granules
Nac03
Stain of eosinophil
Philoxine
Used to detect normal adrenal fxn
Thorn test
Dec eosinoohil under cushing dse
Hyperaldrenalism
Inc eosino but there is an absence of eosino response under addisons dse
Hypoaldrenalism
Inject how many ACTH and how many hours
25 mg of ACTH and wait for 4 hours
How many hours of dfasting
12 hours
50% from base line eosino is
Normal
Value baseline 0-30 mm3 after 4 hours a pt has
Hyperaldrenalism
If pt value remain increase or no change the baseline it is
Hypoaldrenalism
