Lab Test Primary Hemostasis Flashcards
Disease under thrombocytosis
Polycythemia vera
Idiopathic thrombocythemia
CML
Splenectomy
Under thrombocytopenia
Thrombocytopenia purpura Aplastic anemia Acute leukemia Pernicious anemia Gaucher disease
Determination of manual plt ct under direct
Rees ecker
Guy leake
Brecher cronkite
Unopette
Manual plt ct under indirect
Damashek’s
Fonio’s
Olef
Method for platelet estimate 8-20 platelets/field
Wedge smear
Automated
Particle 2-200fl
Marked decreased
0-49,000/ul
Low normal
150,000-199,000/ul
Moderate decreased
50,000-99,000 ul
Normal
200,000- 400,000ul
Marked increases
Above 800,000 ul
Slow dec
100,000-149,000
Slight increase
401,000-599,00 ul
Moderate increase
600,000-800,000 ul
Normal value of plt ct
150,000-400,000 x 10’9/L
Normal plt ct + prolonged BT
Qualitative platelet abnormality
Primary vascular abnormality
Von willebrand syndrome
Low plt ct + normal bt
Autoimmune thrombocytopenia
Low plt ct + prolonged BT
Simultaneous quantitative and qualitative plt deficiency
In vitro test to determine the ability of plt to aggregate with certain agonist
Platelet aggregation Epinephrine Ristocetin ADP Collagen
Platelet rich plasma + agonist
Optical density
Detects in vitro adhesion
Platelet adhesiveness saizmann
Decrease vwd
P rotutine procedure, collection
P collected through glass bead collecting system
Measures the ability of platelet to adhere in glass surface
Platelet adhesiveness
Computation in platelet adhesive
%PA: (PC1-PC2) / PC1 x 100
Nv of vwd
26-30%
Under clot retraction time
Castor Oil
Stefanini
Macfarlane
Formation pf dimplings droplets like serum on the surface of the blood drop
Castor Oil or hirschboeck
Nv pf castor oil
15-45 mins
3-5ml of blod at 37 deg read after 1,2,16,18,24 hours
Stefanini
5,l of blood at 37 deg read after 1 hr
Mcfarlane
Nv of mcfarlane
44-67%
Computation under clot retraction
Vol serum/TV X 100
Measures capillaries to resist pressure correlate with the segree of thrombocytopenia
Capillary resistance test
0-10 few
1+
20-50 multiple on hand and arm
3+
10-20 many
2+
> 50 confluent on hand and arm
4+
In vivo measure of primary hemostasis
Bleeding time
Finger tip or earlobe
Dukes method
Blood pressure cuff at 40 mmhg puncture on forearm
Ivys method
Nv of BT
2-4mins
