Plaelet Studies Flashcards
Increase in platelet ass with disease
Thrombocytopenia
Increase platelet ct but without disease
Thrombocythemia
Nv of platelet
200-400,000/mm3
Factors with in increase platelet ct
Polycythemia vera
After splenectomy
Thrombocythemia
Among with tb
Factors with decrease in platelet ct
Pernicious anemia Aplastic anemia Lesion involving MB Acute leukemia Idiopathic
Method called in light microscopy
Rees and ecjker
Rees and ecker is made up of
Brilliant cresyl blue, na citrate, water
2nd light microscopy in method
Guy and leake’s method
Diluting fluid of 2nd lightmicroscopy
Crystal violet, na citrate and water
Formula of 1st light microscopy
Ply counter x 10 x 200 / 4
Formula of 2!nd light microscopy
Platelet counter x 10 x 200 x 1
Method under phase microscopy
Brencher cronkite Unopette Nygard's Van allen Tocantin's
Most accurate method in phase microscopu
Brecher cronkite
What diluting fluid used in phase microscopy
1% nh4 c204
Nv in phase microscopy
150,000-450,000/mm3
Nv in light microscopy
140,000-340,000/mm3
Life span of platelet
8-11 days
What are the 2 indirect method
Damashek
Fonio
Dikuting fluid of damashek’s method
Brilliant cresyl blue, na citrate, dw, sucrose, formalin
Nv of damashek
500-900,000
Diluting fluid of fonio
14% mg2sO4
Counted by means of electronic particle counter
Semi automated
Counted by means of eptical particle under the principle of dark field microscopy or electronic particle aperture
Fully automated
Solidifying of blood brought about by different coagulation factors. Esp when the blood is taken out from the body
Coagulation
Entire mechanism by which bleeding from an injured blood vessel is spontaneously controlled and stop
Hemostasis
3 general method in coagulation of hemostasis
Bleeding time
Clotting time
Clot retraction time
Nv of dukes method
2-4mins
Nv of ivys method
1-7mins
Puncturing decvuce of ivys method
Bard parker blade, sohygmanometer in 40mmhg
Uses pre warmed NSS with 37 def temp, dip puncture finger until bleeding stops
Copley la litch method
Nv of copley la litch
3-6mins
Patient should abstain taking aspirin in 3 days
Aspirin tolerance test
If more than 6mins of bt in aspirin tolerance
Do not proceed
Do the dukes method for bt in what method
Aspirin tolerance test
Nv of aspirin tolerance test
Difference bet 1st and 2nd should not exceed than 2mins
It test the ability of the capillary under inc hypoxia and hydrostatic pressure
Capillary fragility test
Observe of the presence of petechiae
Capillary fragility test
Get the mid value of of the pt systolic and diastolic pressure, retain those within 5mins and wait for presence of petechia
Rumple leede test
Nv of petechiometer
0-2
The pressure is 200nm
Suction or petechiometer
Time it takes for a fluid blood to solidify after it has been taken out of the body
Coagulation time
Methods under coagulation time
Slide method Capillary tube method Lee and white method Prothrombin time Partial thromboplastin time
Put a drop of blood and fist put every 30 sec, obserbe for fibri trends then stop
Slide method
Nv of capillary tube method under sabraze
3-7mins
Nv of slide method
2-4mins
Uses ordinary capillary tube blue
Dale and laidlaw
Uses special capillary tube which has wider diameter
Sabraze
Whole blood clotting time
Prepare 3 test tube 1ml blood each tube
Tilt every 39secs
Lee and white method
Nv of lee and white method
7-15mins
Use of measure the extrinsic coagulation system and determine coagulation factor 2,5,7,10
Prothrombin time
Prothrombine time uses
Citrate plasma
Nv of prothrombin time
10-12 secs
Measure intrinsic coagulation system ans will measure deficiency of coag factors 8,9,12,11
Partial thromboplastin
