Recombinant DNA technology Flashcards

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1
Q

What is genetic engineering?

A

Transforming organisms using recombinant DNA technology

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2
Q

What is recombinant DNA technology?

A

Genes which code for desirable proteins are placed into organisms using in vivo cloning

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3
Q

How do plants become transformed?

A

The target gene is inserted into the plasmid.
The plasmid is then placed into the bacterium
The bacterium acts as a vector to put the target gene into the plant

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4
Q

What are the two ways which animals become transformed?

A

Either by embryo or egg

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5
Q

How do animals become transformed by an embryo?

A

The genes for the desirable protein are added into an early animal embryo. All of the body cells will then have the gene

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6
Q

How do animals become transformed using the egg of a female?

A

The genes for the desirable protein are added into the egg, so the offspring will then have the gene

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7
Q

Why are promoter regions important?

A

Promoter regions that are only activated in specific cell types can be used to control which part of an animal’s body cells the protein is produced in

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8
Q

What happens if the protein is produced in the wrong cells?

A

It could damage the animal

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9
Q

Why is a higher yield and more nutritious produce a benefit of transformed organisms in agriculture?

A

It can reduce the risk of famine and malnutrition

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10
Q

Why are crops being resistant to pests and droughts a benefit in agriculture?

A

Fewer pesticides which reduces costs and environmental problems with chemicals

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11
Q

How do transformed organisms benefit industry?

A

They are often used in enzymes

Produced in large quantities for less money by transforming organisms

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12
Q

How do transformed organisms benefit medicine?

A

Many drugs and vaccines are produced using transformed organisms
Eg. Human insulin can be cloned from insulin gene rather than obtained from pigs

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13
Q

What are issues with recombinant DNA technology in agriculture?

A
  • Monoculture - farmers only use one type of crop
  • Reduces biodiversity which damages the environment
  • Possibility of superweeds - weeds resistant to herbicides could lead to uncontrolled spread of recombinant DNA with unknown consequences
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14
Q

What are issues with recombinant DNA technology in industry?

A
  • Without proper labelling, some people think they wont have a choice about whether to consume GE food
  • Companies with the technology get bigger and more powerful which may run smaller companies out of business if its harder to compete
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15
Q

What are issues with recombinant DNA technology in medicine?

A
  • Companies who own GE technologies may limit the use of technologies which could save lives
  • It is unethical, eg. designer babies
  • Creates ownership issues
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