Rate Determining Enzymes of Metabolic Processes

Question 1

glycolysis

Answer 1

PFK-1: +AMP +F-2,6-BP; -ATP, -citrate

Question 2

gluconeogensis

Answer 2

F-1,6-BP: -AMP, -F-2,6-BP

Question 3

TCA cycle

Answer 3

isocitrate dehydrogenase: + ADP; - ATP, -NADH

Question 4

glycogenesis

Answer 4

glycogen synthase: +G-6-P +insulin +cortisol; -epi, -glucogon

Question 5

glycogenolysis

Answer 5

glycogen phosphorylase: +epi +glucagon +AMP; -G-6-P, -insulin, -ATP

Question 6

HMP shunt

Answer 6

G6PD: +NADP, -NADPH

Question 7

de novo pyrimidine synthesis

Answer 7

CPS II: +ATP, +PPRP, -NADPH

Question 8

de novo purine synthesis

Answer 8

glutamine-PRPP amidotransferase: -AMP, -IMP, -GMP

Question 9

urea cycle

Answer 9

CPS I: +N-acetylglutamate

Question 10

FA synthesis

Answer 10

acetly-CoA carboxylase (ACC): +insulin, +citrate; -glucagon, -palmitoyl-CoA

Question 11

FA oxidation

Answer 11

carnitine acyltransferase I: -malonyl-CoA

Question 12

ketogenesis

Answer 12

HMG-CoA synthase

Question 13

cholesterol synthesis

Answer 13

HMG-CoA reductase: +insulin +thyroxin; -glucagon - cholesterol