quiz for 4-22-15

Question 1

what is the removal and destruction of all viable microbes

Answer 1

sterilization

Question 2

what is the destruction of vegetative pathogens on inanimate objects

Answer 2

disinfection

Question 3

what is the reduction of microbial population to the level considered safe by the department of health

Answer 3

sanitization

Question 4

what is used on inanimate objects such as bench tops, counter tops

Answer 4

disinfectants

Question 5

what is used on living tissue, something you could use on a cut

Answer 5

antiseptic

Question 6

any chemical that can inhibit microbes from growing in or on the body

Answer 6

chemotherapeutic agent

Question 7

a substance produced by microbes that can inhibit growth of other microbes.

Answer 7

antibiotic

Question 8

a test that is widely used for testing the sensitivity of bacteria to antimicrobials

Answer 8

Kirby-Bauer Disk Diffusion test

Question 9

what is the method of streaking the plate for the Kirby-Bauer method

Answer 9

using a swab and applying it in the “lawn” method

Question 10

what is the area surrounding the antibiotic disks that are free from growth called

Answer 10

the zone of inhibition

Question 11

how is the zone of inhibition measured

Answer 11

in mm

Question 12

How is the agar prepared in the Kirby-Bauer Method

Answer 12

it must be 4mm thick and the media must be Mueller-Hinton agar

Question 13

How much bacteria is applied to the agar in the Kirby-Bauer method

Answer 13

0.5 McFarland Turbidity Standard

Question 14

How long is the plates incubated for the Kirby-Bauer Method

Answer 14

18-24 hours

Question 15

How old can the culture for set up be for the Kirby-Bauer Method

Answer 15

2-6 hours old

Question 16

what is the incubation temperature for the Kirby-Bauer Method

Answer 16

35 degrees Celsius +or- 2 degrees

Question 17

how are the results recorded for the Kirby-Bauer Method

Answer 17

as either resistant, intermediate or sensitive

Question 18

what is the lowest concentration of antibiotic that prevents bacterial growth known as

Answer 18

Minimal inhibitory concentration

Question 19

an antibiotic that works against a large range of bacteria is known as

Answer 19

broad spectrum

Question 20

an antibiotic that works against a large range of bacteria is known as

Answer 20

broad spectrum

Question 21

an antibiotic that works against a small range of bacteria is known as

Answer 21

narrow spectrum

Question 22

term given to an antibiotic that kills bacteria

Answer 22

bactericidal

Question 23

term given to an antibiotic that inhibits reproduction

Answer 23

bacteriostatic

Question 24

give an example of how antibiotics work

Answer 24

1. inhibition of cell wall
2. breakdown of cell membrane
3. inhibition of structure and function of DNA and RNA
4. inhibition of protein synthesis
5. blocks a key metabolic pathway