quiz for 4-22-15 Flashcards
what is the removal and destruction of all viable microbes
sterilization
what is the destruction of vegetative pathogens on inanimate objects
disinfection
what is the reduction of microbial population to the level considered safe by the department of health
sanitization
what is used on inanimate objects such as bench tops, counter tops
disinfectants
what is used on living tissue, something you could use on a cut
antiseptic
any chemical that can inhibit microbes from growing in or on the body
chemotherapeutic agent
a substance produced by microbes that can inhibit growth of other microbes.
antibiotic
a test that is widely used for testing the sensitivity of bacteria to antimicrobials
Kirby-Bauer Disk Diffusion test
what is the method of streaking the plate for the Kirby-Bauer method
using a swab and applying it in the “lawn” method
what is the area surrounding the antibiotic disks that are free from growth called
the zone of inhibition
how is the zone of inhibition measured
in mm
How is the agar prepared in the Kirby-Bauer Method
it must be 4mm thick and the media must be Mueller-Hinton agar
How much bacteria is applied to the agar in the Kirby-Bauer method
0.5 McFarland Turbidity Standard
How long is the plates incubated for the Kirby-Bauer Method
18-24 hours
How old can the culture for set up be for the Kirby-Bauer Method
2-6 hours old
what is the incubation temperature for the Kirby-Bauer Method
35 degrees Celsius +or- 2 degrees
how are the results recorded for the Kirby-Bauer Method
as either resistant, intermediate or sensitive
what is the lowest concentration of antibiotic that prevents bacterial growth known as
Minimal inhibitory concentration
an antibiotic that works against a large range of bacteria is known as
broad spectrum
an antibiotic that works against a large range of bacteria is known as
broad spectrum
an antibiotic that works against a small range of bacteria is known as
narrow spectrum
term given to an antibiotic that kills bacteria
bactericidal
term given to an antibiotic that inhibits reproduction
bacteriostatic
give an example of how antibiotics work
- inhibition of cell wall
- breakdown of cell membrane
- inhibition of structure and function of DNA and RNA
- inhibition of protein synthesis
- blocks a key metabolic pathway
