class notes 2-4-15 Flashcards
what is the ability of a lens system to distinguish objects as separate entities that are very small and very close together.
resolution
what is determined by the quality of the lens and the wavelength of light used
resolution
what is the ability of making a small object appear larger
magnification
what is described as, when magnification increases the focal point remains the same as
Parfocal lens
what is described as, when focusing up and down as magnification increases the depth of field decreases ( seeing all 3 layers at once)
Depth of field
what is described as, when magnification increases the center of the field of view remains the same
Parcentral
what are the 3 properties of microscopy
magnification
contrast
resolving power
what is the degree to which an image stands out against its background?
contrast
what is the amount that the image of an object is enlarged?
magnification
what color are bacteria?
colorless
the ability of a lens to separate between small objects that are close together
resolving power
when things are not resolved this is known as
empty magnification
when looking through the oculars, the area of the slide that can be observed is known as
the field of view
when changing from one objective to another, the specimen will remain very nearly in focus, is known as
Parfocal
when changing objectives the specimen will stay in the center of the field of view ( when focused properly) is known as
Parcentral
what part of the microscope directs and concentrates the light
condenser lenses
to be able to see objects through a microscope, what must be either reflected from the object or transmitted through the object
light
what type of light is used during most of your observations with the microscope
transmitted light
name the 4 properties of light
reflection
refraction
transmission
absorption
when light bounces back from the object, the particular wavelengths reflected back to the eye determine the perceived color is known as what property of light
reflection
what property of light refers to the passage of light through the object
transmission
what is the pathway of light?
light source condenser lens through the specimen objective lens ocular lens
if light rays don’t pass through or bounce off the object but are taken up by the objects.
absorption
the absorbed light rays are reemitted as longer wavelengths is a phenomenon known as
fluorescence
the bending of light as it passes from one medium to another of a different density
refraction
name the 2 types of microscopes
light microscope
electron microscope
name the 2 types of light microscopes
stereo dissecting light
compound light
name the 2 types of electron microscopes
scanning electron microscope
transmission electron microscope
which of the light microscopes has 3D view and can view larger specimens
stereo dissecting light
which of the light microscopes has a 2D view and can view smaller specimens in thin sections
compound light microscope
which of the electron microscopes has a 3D view of the external surfaces of the specimen
scanning electron microscope
which of the electron microscopes has a 2D view of the internal structures of the specimen
transmission electron microscope
what is the maximum magnification of a light microscope
2,000x
what is the maximum magnification of an electron microscope
100,000-1,000,000
what type of specimens are viewed with a light microscope ( alive or dead)
both may be viewed
what type of specimens are viewed with an electron microscope ( alive or dead)
non living
which of the 2 types of microscopes can the specimens be viewed in color
light microscope
what is obtained when you multiply the power of the ocular times the power of the objective?
total magnification
