quiz 3-3-15

Question 1

what can be measured either quantitatively or qualitatively

Answer 1

bacterial growth

Question 2

this device electronically counts cells as they pass through it

Answer 2

coulter counter

Question 3

this device manually counts cells with a microscope and has a disadvantage not being able to discriminate between dead and alive cells

Answer 3

Petroff Hausser counting chamber

Question 4

what is the method of counting bacteria in which you spread the bacteria on a plate with a sterile hockey stick and incubate it?

Answer 4

spread method

Question 5

what is the method of counting bacteria in which you pour molten agar medium into a plate and the bacterial colonies grow inside and on top of the agar?

Answer 5

pour method

Question 6

Bacteria divide by what method?

Answer 6

binary fission

Question 7

what phase of growth is when the cells are metabolically active and not reproducing?

Answer 7

lag phase

Question 8

what phase of growth is when the cells are reproducing at an exponential rate?

Answer 8

log phase

Question 9

what phase of growth is when the cells reproduction is equal to the rate of the cells dying?

Answer 9

stationary phase

Question 10

what phase of growth is when the cells are dying off at an exponential rate?

Answer 10

death phase

Question 11

what it the time it takes for the population to double; which is genetically determined?

Answer 11

generation time

Question 12

what is the gas requirement category for a microbe that cannot grow without oxygen?

Answer 12

obligate aerobe

Question 13

what is the gas requirement category for a microbe that requires oxygen for its metabolism and is capable of growth in the absence of it?

Answer 13

facultative anaerobe

Question 14

what is the gas requirement category for a microbe that does not grow at normal atmospheric concentrations of oxygen but requires a small amount of it ( 1-15%)?

Answer 14

microaerophile

Question 15

what is the gas requirement category for a microbe that lacks the enzyme for processing toxic oxygen, they cannot tolerate only free oxygen, they do not use oxygen for respiration and are not able to grow in the presence of oxygen?

Answer 15

obligate anaerobe

Question 16

what is the gas requirement category for a microbe that does not utilize oxygen but can survive and grow in its presents?

Answer 16

aerotolerant anaerobe

Question 17

what is the gas requirement category for a microbe that grows best at higher carbon dioxide tensions (3-10%)?

Answer 17

capnophiles

Question 18

what is the name of the medium that creates a reduced environment in the semi-soft agar, with a small amount of oxygen diffusing in from the top?

Answer 18

thioglycollate

Question 19

what cell wall is 60-90% peptidoglycan?

Answer 19

gram +

Question 20

what cell wall is 10-20% peptidoglycan?

Answer 20

gram -

Question 21

which cell wall retains the primary dye?

Answer 21

gram +

Question 22

which cell wall type does the decolorizer remove the primary dye?

Answer 22

gram -

Question 23

which cell wall type picks up the counterstain?

Answer 23

gram-

Question 24

which cell wall type has a lipopolysaccharide layer?

Answer 24

gram-

Question 25

which cell wall type has teichoic acid and lipoteichoic acids?

Answer 25

gram +

Question 26

what is the result that has both gram - and gram + results

Answer 26

gram variable

Question 27

what would cause a gram variable result?

Answer 27

age of the culture
technique
genetics
medium

Question 28

what is the result, in which the bacteria doesn’t pick up either the primary or the counterstain?

Answer 28

gram non reaction

Question 29

what would cause a gram non reaction result?

Answer 29

waxy lipids in the cell wall

example: mycobacteria, norcardia, lepra