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Microbiology lab > quiz 4-15-15 > Flashcards

quiz 4-15-15 Flashcards

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1
Q

what type of charge do acid dyes have

A

a negative charge

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2
Q

what is the waxy lipid layer that some bacteria cells have in their cell walls

A

mycolic acid

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3
Q

what is the purpose of the mycolic acid

A

to protect the bacteria from drying out, protect it from disinfectants and the immune system

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4
Q

which cells do the safranin color, vegetative or capsules

A

vegetative cells

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5
Q

give an example of an acid fast bacteria

A

mycobacteria and nocardia, they are both rod shaped

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6
Q

in what phase of TB is the person coughing up bloody mucus, having night sweats, and increased fever

A

the active phase

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7
Q

in what phase of TB can become full blown in immuno compromised people, that have been exposed to the bacteria but initially were not showing any signs

A

latent TB

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8
Q

what is the oxygen requirement, arrangement and motility of mycobacterium

A

obligate aerobe, non-motile, rod shaped

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9
Q

how long can organisms remain alive in dried sputum from mycobacterium

A

6 months

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10
Q

how much of the worlds population has TB

A

1/3 of the world

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11
Q

what is the the name of the test that uses heat fixed and time to force the stain into the waxy cell walls

A

ziehl-neelsen acid fast stain

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12
Q

in the ziehl-neelsen acid fast stain what is the decolorizor used

A

acid alcohol

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13
Q

in the ziehl-neelson acid fast stain what is the name of the primary stain

A

Carbol fuchsin

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14
Q

in the ziehl-neelson acid fast stain what is the counterstain

A

methylene blue

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15
Q

how long is the slide heated for the ziehl-neelson acid fast stain

A

5 minutes

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16
Q

after the primary stain is applied and the decolorizer is used, what color are the non acid fast bacteria in the ziehl-neeson acid fast stain

A

pink

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17
Q

after the counterstain is applied in the ziehl-neelson acid fast stain, what color are the non-acid fast bacteria

A

blue

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18
Q

what color would mycobacteria be after the ziehl-neelson acid fast stain was complete

A

they would be blue, because they are non acid fast, they resist the decolorizer

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19
Q

what is used during the kinyoun method to force the carbol fuchsin into the waxy cell wall

A

it uses a wetting agent, and a higher concentration of the carbol fuchsin

20
Q

what is the decolorizer in the kinyoun cold method

A

acid alcohol

21
Q

what is the counterstain in the kinyoun acid fast stain

A

brilliant green

22
Q

what color are the acid fast bacteria after applying the carbol fuchsin, during the kinyoun acid fast method

A

pink

23
Q

what color are the non acid fast bacteria after the kinyoun acid fast stain

A

brilliant green

24
Q

what is the route of transmission for the trypanosoma brucei

A

bite from a tsetse fly

25
Q

what is the mode of transmission for trypanosoma cruzi

A

bite from the kissing bug

26
Q

what is the motility of trypanosoma cruzi and trypanosoma brucei

A

flagella

27
Q

what is the infective stage for trypanosoma brucei and cruzi

A

trypomastigote

28
Q

what is the definative host in the trypanosoma brucei and trypanosoma cruzi

A

the human

29
Q

what is the intermediate host in trypanosoma brucei

A

the tsetse fly

30
Q

what is the intermediate host for trypanosoma cruzi

A

the kissing bug

31
Q

what disease is caused by the trypanosoma brucei

A

african sleeping sickness

32
Q

what is the sickness that is caused by tropanosoma cruzi

A

chagas disease- south american

33
Q

what is the mode of transmission of the leishmania donovani

A

flagella

34
Q

what is the motility of the leishmania donovani

A

flagella

35
Q

what is the infective stage of the leishmania donovani

A

promastigote

36
Q

what is the definative host of the leishmania donovani

A

the human

37
Q

what is the intermediate host of leishmania donovani

A

the sand fly

38
Q

what is the disease caused by leishmania donovani

A

kala azar

39
Q

what is the mode of transmission for cryptosporidium parvum

A

fecal oral ingestion of the oocyst

40
Q

what is the motility of the cryptosproridium parvum

A

none they are amicomplexa

41
Q

what is the infective stage of the cryptosporidium parvum

A

oocyst

42
Q

what is the host of the cryptosporidium parvum

A

humans and other animals

43
Q

what disease is caused by cryptosporidium parvum

A

cryptosproidiosis

44
Q

what is the name of the mode of transmission for the toxoplasma gondii

A

ingestion of the cyst transferred from the mother to the fetus

45
Q

what type of charge do capsules have

A

a negative charge

Microbiology lab (12 decks)

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