quiz 4-15-15 Flashcards
what type of charge do acid dyes have
a negative charge
what is the waxy lipid layer that some bacteria cells have in their cell walls
mycolic acid
what is the purpose of the mycolic acid
to protect the bacteria from drying out, protect it from disinfectants and the immune system
which cells do the safranin color, vegetative or capsules
vegetative cells
give an example of an acid fast bacteria
mycobacteria and nocardia, they are both rod shaped
in what phase of TB is the person coughing up bloody mucus, having night sweats, and increased fever
the active phase
in what phase of TB can become full blown in immuno compromised people, that have been exposed to the bacteria but initially were not showing any signs
latent TB
what is the oxygen requirement, arrangement and motility of mycobacterium
obligate aerobe, non-motile, rod shaped
how long can organisms remain alive in dried sputum from mycobacterium
6 months
how much of the worlds population has TB
1/3 of the world
what is the the name of the test that uses heat fixed and time to force the stain into the waxy cell walls
ziehl-neelsen acid fast stain
in the ziehl-neelsen acid fast stain what is the decolorizor used
acid alcohol
in the ziehl-neelson acid fast stain what is the name of the primary stain
Carbol fuchsin
in the ziehl-neelson acid fast stain what is the counterstain
methylene blue
how long is the slide heated for the ziehl-neelson acid fast stain
5 minutes
after the primary stain is applied and the decolorizer is used, what color are the non acid fast bacteria in the ziehl-neeson acid fast stain
pink
after the counterstain is applied in the ziehl-neelson acid fast stain, what color are the non-acid fast bacteria
blue
what color would mycobacteria be after the ziehl-neelson acid fast stain was complete
they would be blue, because they are non acid fast, they resist the decolorizer
what is used during the kinyoun method to force the carbol fuchsin into the waxy cell wall
it uses a wetting agent, and a higher concentration of the carbol fuchsin
what is the decolorizer in the kinyoun cold method
acid alcohol
what is the counterstain in the kinyoun acid fast stain
brilliant green
what color are the acid fast bacteria after applying the carbol fuchsin, during the kinyoun acid fast method
pink
what color are the non acid fast bacteria after the kinyoun acid fast stain
brilliant green
what is the route of transmission for the trypanosoma brucei
bite from a tsetse fly
what is the mode of transmission for trypanosoma cruzi
bite from the kissing bug
what is the motility of trypanosoma cruzi and trypanosoma brucei
flagella
what is the infective stage for trypanosoma brucei and cruzi
trypomastigote
what is the definative host in the trypanosoma brucei and trypanosoma cruzi
the human
what is the intermediate host in trypanosoma brucei
the tsetse fly
what is the intermediate host for trypanosoma cruzi
the kissing bug
what disease is caused by the trypanosoma brucei
african sleeping sickness
what is the sickness that is caused by tropanosoma cruzi
chagas disease- south american
what is the mode of transmission of the leishmania donovani
flagella
what is the motility of the leishmania donovani
flagella
what is the infective stage of the leishmania donovani
promastigote
what is the definative host of the leishmania donovani
the human
what is the intermediate host of leishmania donovani
the sand fly
what is the disease caused by leishmania donovani
kala azar
what is the mode of transmission for cryptosporidium parvum
fecal oral ingestion of the oocyst
what is the motility of the cryptosproridium parvum
none they are amicomplexa
what is the infective stage of the cryptosporidium parvum
oocyst
what is the host of the cryptosporidium parvum
humans and other animals
what disease is caused by cryptosporidium parvum
cryptosproidiosis
what is the name of the mode of transmission for the toxoplasma gondii
ingestion of the cyst transferred from the mother to the fetus
what type of charge do capsules have
a negative charge
