quiz 4 Flashcards
oil rig
o - oxidation
i-is
l- losing H+
r=reduction
i=is
g= gaining H+
NADH is a
reduction reaction
-NAD —- NADH
by-product of NADH pathways
glycolysis, PDH, Krebs and beta oxidation
only glycolysis is outside of the
mitochondria
NADH goes to
mitochondria and ETC and ATP is made
FADH is a
reduction reaction
-FAD —- FADH2
by-product of FADH pathways
krebs and beta-oxidation
all FADH2 is produced inside
mitochondria
FADH2 goes to
mitochondria and ETC
-ATP is made
how do we get glycolytic NADH’s into the mitochondria and ECT
malate Asparte Shuttle
glycerol-phosphate shuttle
ETC is a
oxidative reaction
1 NADH is mitochondrial matrix =
2.5 ATP because of ETC
1 FADH in the mitochondrial matrix
1.5 ATP because of ETC
Reducing equivalents transfers
H and e into mitochondria]
-NAD stays outside
glycerol phosphate shuttle
transfers H and E into mitochondria to make ATP
-NAD stays outside
- NADH. to FADH
- to ETC in mitochondria
mitochondrial pathways
- PDH
- Beta Oxidation
- Krebs Cycle
- ETC
pyruvate dehydrogenase
links glycolysis and kreb cycle together
pyruvate is produced by glycolysis in
in the sarcoplasm
PDH converts pyruvate
to Acetyl-CoA
after PDH converts pyruvate to Acetyl-CoA
- uses CoA - substrate
- Makes NADH (can be used in the ETC)
- Makes CO2
- Acetyl-CoA enters Kreb cycle
a substrate of kreb cycle is
acetyl CoAQ
Kreb cycle does not have
O2
krebs cycle: 1 molecule of glucose =
= 2 turns of krebs cycle
-2 pyruvate then 2 acetyl CoA
substrates
- Acetyl CoA
- GDP/ADP
- 3 NAD
- 1 FAD
Products
- Oxaloacetate (OAA)
- GTP/ATP
- 3 NADH
- 1 FADH2
counting ATP for PDH–>pyruvate–>Acetyl CoA
1 NADH x 2.5 ATP = 2.5 ATP
3 NADH for one spin krebs cycle
3 NADH x 2.5 ATP = 7.5
1 FADH2 for one spin krebs cycle
1 FADH2 X 1.5 ATP = 1.5 ATP
1 GTP For one spin Krebs cycle
1 ATP
how many total ATP for one spin of Kreb cycle
10
rate-limiting enzyme for Krebs cycle
Isocitrate dehydrogenase
isocitrate dehydrogenase is inhibited by
ATP and NADH
isocitrate dehydrogenase is activated by
ADP and NAD
kreb cycle occurs in the
mitochondria matrix
end molecule of kreb cycle is
OAA
1 molecule glucose =
2 pyruvate and 2 acetyl CoA
can i keep selling sex for money
- citrate
- isocitrate
- Alpha- ketogluterate
- succinyl CoA
- succinate
- Fumarate
- Malate
- Oxaloacetate
slow path ways- Beta Oxidation occurs in the
mitochondrial matrix
beta-oxidation breaks
breaks fatty acids down to Acetyl CoA
-no O2
NADH produced by beta-oxidation
goes to ETC
fatty acids is a
substrate of beta oxidation
acetyl CoA is a
substrate going into the kreb cycle
substrates for beta-oxidation
fatty acids, CoA, FAD, NAD
products of beta-oxidation
Acetyl- CoA, FADh2, NADH
integration of Beta oxidation and bioenergetics
acetyl CoA and reducing equivalents (NADH, FADH)
slow pathways - ETC located in
mitochondrial matrix and inner mitochondrial membrane
products of ETC
- NAD
- FAD
- H2O
- ATP
ETC pumps H into intermembrane space
building gradient causes H to flow back into matrix via ATP synthase
no O2 present
ETC unable to perform
O2 dependent ATP synthesis
three pathways used in glucose oxidation
- glycolysis
- krebs cycle
- ETC
- PDH
end products of incomplete oxidation of glucose
pyruvate and lactate
-during high intensity
end products of complete oxidation of glucose
pyruvate to the mitochondria
-during low intensity
-substrate level phosphorylation =ATP
glycolysis RE
NADH
Linker enzyme of glycolysis
PDH, NADH, CO2, Acetyl -CoA
ETC
oxidative phosphorylation to make ATP, NAD and FADH
Krebs cycle RE
NADH and FADH2, CO2
substrate level phosphorylation =ATP
what shuttle transport glucose
GLUT 4
3 main bioenergetics pathways used in fat oxidation
- krebs cycle
- ETC
- Beta oxidation
all bioenergetics use ____ as fuel are found within the _____
fat and mitochondria
possible fuels: blood fatty acids
dietary and adipose tissue and IMTG
Mobilizing fatty acids from stored trig through
lipolysis (HSL)
-if adipose- then transport muscle
-if IMTG, then already in muscle
events leading to using fat as fuel
- mobilize
- transport fatty acids to muscle
- transport fatty acids into muscle
- Activation
- transport into mitochondria
- oxidize
transport fatty acids to muscle
albumin
-if IMTG then no albumin transport
Transport fatty acids into the muscle cell
FATPs
FABP needed as a chaperone in cell (fatty acid binding protein)
activation of fatty acids
Acetyl - CoA synthase uses ATP and adds CoA to fatty acids
transport fatty acids into mitochondria
CPTS, acetyl -coA activates fatty acid and requires 2 ATP equivalents
oxidize fatty acids
beta-oxidation (catabolic)
-makes 2- C acetylCoA from fatty acid
RE - NADH And FADH
ETC uses NADH and FADH from
beta oxidation and kreb cycle
how many times does beta-oxidation “spin”
of carbons/2 - 1
how many actetly coA we get from fatty acids
of carbon/2
beta oxidation =NADH and FADH
1 per spin
FATPs increase the transport of blood FFA and increase amount of FFA due to
HSL
low glycogen states =
increase use of protein as fuel