Q1: Factors Affecting Enzymatic Activity Flashcards
The enzymes become denatured if the temperature is _______ compared to its optimum temperature.
Too High
What happens when an enzyme changes shape?
Reaction slows down
True or False
Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions that can cause an enzyme to lose its ability to bind to a substrate.
TRUE
5 Determinants of the Factors Affecting Enzyme Activities
Temperature, pH, Substrate Concentration, Enzyme Concentration, and Effect of Activators
Raising temperature generally __________ a reaction, and lowering temperature __________ a reaction
Speeds up, Slows down
extreme high temperatures can cause an enzyme to _________ and stop working
Denature
TRUE OR FALSE
Most enzymes have an optimum temperature (temperature at which a procedure is best carried out on the action of an enzyme), near normal body temperature at which they catalyze a reaction most rapidly.
TRUE
pH abbreviation for ______ or ______.
Power of Hydrogen/Potential of Hydrogen
_________ pH values can cause enzymes to denature.
Extreme
TRUE or FALSE
Small pH changes does not affect the electrical charges on various chemical groups in enzyme molecules, thereby altering the enzyme’s ability to bind its substrate and catalyze a reaction.
FALSE
Even small pH changes can alter the electrical charges on various chemical groups in enzyme molecules, thereby altering the enzyme’s ability to bind its substrate and catalyze a reaction.
Enzymes catalyze a reaction most rapidly at an _________, near _______.
optimum range, neutral
Increasing substrate concentration, _________ the rate of reaction to a certain point.
Increases
TRUE OR FALSE
Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.
True
At the _____________, the reaction will not speed up, no matter how much additional substrate is added.
Saturation Point
The graph of reaction rate is plateauing.
Substrate Concentration