Q1: Factors Affecting Enzymatic Activity Flashcards

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1
Q

The enzymes become denatured if the temperature is _______ compared to its optimum temperature.

A

Too High

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2
Q

What happens when an enzyme changes shape?

A

Reaction slows down

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3
Q

True or False

Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions that can cause an enzyme to lose its ability to bind to a substrate.

A

TRUE

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4
Q

5 Determinants of the Factors Affecting Enzyme Activities

A

Temperature, pH, Substrate Concentration, Enzyme Concentration, and Effect of Activators

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5
Q

Raising temperature generally __________ a reaction, and lowering temperature __________ a reaction

A

Speeds up, Slows down

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6
Q

extreme high temperatures can cause an enzyme to _________ and stop working

A

Denature

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7
Q

TRUE OR FALSE

Most enzymes have an optimum temperature (temperature at which a procedure is best carried out on the action of an enzyme), near normal body temperature at which they catalyze a reaction most rapidly.

A

TRUE

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8
Q

pH abbreviation for ______ or ______.

A

Power of Hydrogen/Potential of Hydrogen

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9
Q

_________ pH values can cause enzymes to denature.

A

Extreme

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10
Q

TRUE or FALSE

Small pH changes does not affect the electrical charges on various chemical groups in enzyme molecules, thereby altering the enzyme’s ability to bind its substrate and catalyze a reaction.

A

FALSE

Even small pH changes can alter the electrical charges on various chemical groups in enzyme molecules, thereby altering the enzyme’s ability to bind its substrate and catalyze a reaction.

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11
Q

Enzymes catalyze a reaction most rapidly at an _________, near _______.

A

optimum range, neutral

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12
Q

Increasing substrate concentration, _________ the rate of reaction to a certain point.

A

Increases

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13
Q

TRUE OR FALSE

Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.

A

True

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14
Q

At the _____________, the reaction will not speed up, no matter how much additional substrate is added.

A

Saturation Point

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15
Q

The graph of reaction rate is plateauing.

A

Substrate Concentration

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16
Q

Increasing enzyme concentration will _______ the reaction, as long as there is substrate available to bind to.

A

Speed up

17
Q

TRUE or FALSE

The higher the concentration of an enzyme the greater should be the initial reaction rate. This will last as long as there is an infinite amount of substrate present.

A

TRUE

18
Q

The graph of reaction rate is linear

A

Enzyme Concentration

19
Q

The graph of reaction rate is Bell-shaped.

A

pH

20
Q

TRUE or FALSE

Some of the enzymes do not require certain inorganic metallic cations, like Mg2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+, Na+, K+ etc., for their optimum activity. Rarely, anions are also needed for enzyme activity, e.g. a chloride ion (CI–) for amylase.

A

FALSE

Some of the enzymes require certain inorganic metallic cations, like Mg2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+, Na+, K+ etc., for their optimum activity. Rarely, anions are also needed for enzyme activity, e.g. a chloride ion (CI–) for amylase.