Proteins Flashcards

1
Q

What are the basic building blocks of proteins?

A

Proteins are built of amino acids. There are around 20 types of amino acids found in all living organisms.

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2
Q

Proteins vary hugely in function. Give an example of proteins and their function.

A

An example of a protein is haemoglobin (the thing responsible for blood being red) it is an iron-containing protein that plays a role in the transportation of oxygen in the blood. Another protein: fibrin forms a mesh of threads over the surface of wounds trapping blood cells and forming scabs.

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3
Q

What is the test for protein?

A

Biuret solution is used to identify the presence of protein. Biuret reagent is a blue solution that, when it reacts with protein, will change color to pink-purple.

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4
Q

About the 20 types of amino acids

A

All 20 of the amino acids have the same general structure. They contain a carboxyl group (cooh) an amine group (NH2) and an R group (the R group is what decides what amino acid it is). They also contain a Hydrogen atom

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5
Q

What is an R group?

A

The thing that differentiates the amine groups from each other.

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6
Q

What is a peptide bond?

A

A chemical bond formed between two amino acids as a result of condensation.

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7
Q

What do you get when you cross two amino acids?

A

A dipeptide (two amino acids joined by a peptide bond)

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8
Q

What type of reaction do amino acids join to form a dipeptide?

A

A condensation reaction forms a dipeptide and removes hydrogen but creates a molecule of water

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9
Q

What is primary structure?

A

The sequence of amino acids in a polypeptide

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10
Q

What is secondary structure?

A

The shape the polypeptide chain folds into, such as alpha helix and beta pleated sheet

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11
Q

What is tertiary structure?

A

Gives a protein the characteristic complex, three-dimensional shapes that are closely related to its function.

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12
Q

Other than the R group what influences a protein’s shape?

A

The order of amino acids determines the protein. Also called the primary structure.

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13
Q

Why is the tertiary structure important?

A

Tertiary structure is important because it’s closely related to the function of the protein. and the different types of bond between different amino acids which maintain the shape.

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14
Q

What are the three types of bonds in proteins?

A

Hydrogen bonds
Ionic bonds
Disulfide bridges

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15
Q

Define and explain Hydrogen bonds

A

Hydrogen bonds form between R-groups of a variety of amino acids. They are weak and easily broken but there are many of them

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16
Q

Define and explain Ionic bonds

A

Ionic bonds form between amino acids with a positive charge and an amino acid with a negative charge. They are weak and easily broken.

17
Q

Define and explain Disulfide bridges

A

Disulphide bridges form between amino acids that contain sulphur in their R-groups. They are strong covalent bonds and are less easily broken than Ionic bonds and hydrogen bonds

18
Q

What is a quarternary structure?

A

The quarternary structure is when proteins are made up of more than one polypeptide chain