Methods of studying cells Flashcards
What are the differences in magnification and resolution between the Electron Microscope and Light (optical) Microscope
How do you calculate size of image
Magnification X Size of real object = Size om image
What are the conversion for milimetres to micrometres to nanometres
What is resolution
The ability to distinguish two seperate dots close together
What is magnification
it is how much bigger the image is than the actual specimen
after centrifugation what is the mass at the bottom
pellet
after centrifugation what is the liquid at the top
supernatent
What are the pros of using the electron microscope
you can see more detailed and or smaller things
what are the cons of the electron microscope
they have to be dead cells as it is ina vaccum
it also is black and white
not accessible as it is large and expensive
what are the pros of light microscopes
accessible
coloured
what are the cons of the light microscope
low resolution and magnification
what is a buffer solution
a solution whichkeeps the pH the same
what is an isotonic solution
a solution which stops reactions from happening
