PPT NI BASAS

Question 1

The objective lens
forms the magnified

Answer 1

real image

Question 2

image is
a product of the
separate magnifying
powers of the two
lenses

Answer 2

Total magnification

Question 3

Numerical aperture of lens ranges from

Answer 3

0.1 to 1.25

Question 4

formula for quantifying resolution

Answer 4

RP = wavelength of light in nm/2x numerical aperture of objective lens

Question 5

Oil immersion objectives resolution is

Answer 5

0.2

Question 6

In order to get the best resolution possible with a
light microscope, you would want:

A. Long wavelengths of light

B. Short wavelengths of light

C. The wavelength doesn’t affect resolution

Answer 6

B

Question 7

when a specimen is
darker than surrounding field and used used for live and
preserved stained specimens, what type of microscope is this

Answer 7

Bright-field

Question 8

best for observing
intracellular structures

Answer 8

phase contract

Question 9

this microscope is useful in diagnosing
infections

Answer 9

fluorescence microscope

Question 10

electron micro scopes magnification is about

Answer 10

5,000X and 1,000,000X

Question 11

Darker areas represent
thicker, denser parts
and lighter areas
indicate more
transparent, less dense
parts. what type of microscope is this

Answer 11

transmission

Question 12

allow examination of characteristics of live
cells: size, motility, shape, and arrangement

Answer 12

wet mounts and hanging drop mounts

Question 13

are made by drying and
heating a film of specimen.

Answer 13

fixed mounts

Question 14

cationic,
positively charged
chromophore

Answer 14

basic dyes

Question 15

surfaces of microbes are
negatively charged and
attract basic dyes

Answer 15

positive staining

Question 16

anionic,
negatively charged
chromophore

Answer 16

acidic dyes

Question 17

microbe repels dye, the
dye stains the
background

Answer 17

negative staining

Question 18

one dye is used; reveals
shape, size, and arrangement

Answer 18

simple stains

Question 19

use a primary stain and a
counterstain

Answer 19

differential stains

Question 20

reveal certain cell parts not
revealed by conventional methods: capsule and
flagellar stains

Answer 20

structural stains

Question 21

what are the 6 I of culturing microbes

Answer 21

Inoculation
Isolation
Incubation
Inspection
Information gathering
Identification

Question 22

If an individual bacterial cell is separated from other
cells and has space on a nutrient surface,

Answer 22

isolation

Question 23

it is the Checking for contaminants (unknown or unwanted
microbes) in the culture.

Answer 23

inspection

Question 24

Media is classified into 3 properties

Answer 24

Physical state
Chemical composition
Functional type

Question 25

Provides framework to hold
moisture and nutrients most commonly used
solidifying agent

Answer 25

Agar

Question 26

what are the 2 most commonly used na media

Answer 26

Nutrient broth
Nutrient agar

Question 27

chemical contents of media

Answer 27

Synthetic
Complex
General purpose media
Enriched media

Question 28

contains pure organic and inorganic
compounds in an exact chemical formula

Answer 28

synthetic

Question 29

contains at least
one ingredient that is not chemically definable

Answer 29

Complex

Question 30

grows a broad range
of microbes, usually nonsynthetic

Answer 30

general purpose media

Question 31

contains complex organic
substances such as blood, serum, hemoglobin, or

Answer 31

enriched media

Question 32

contains
one or more
agents that
inhibit growth of
some microbes
and encourage
growth of the
desired
microbes

Answer 32

selective

Question 33

contains a substance
that absorbs oxygen
or slows penetration
of oxygen into
medium; used for
growing anaerobic
bacteria

Answer 33

Reducing media

Question 34

contains sugars that
can be fermented,
converted to acids,
and a pH indicator to
show this reaction

Answer 34

carbohydrate fermentation