Post Transcriptional processing and Translation Flashcards

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1
Q

post transcriptional processing of RNA occurs in …

A

both eukaryote and prokaryotic cells.
in prokaryotic cells, rRNA and tRNA go through it , but mRNA is directly translated into protein.
in eukaryotes each RNA type undergoes post transcriptional processing , which allows for further gene regulation
in eukaryotes , this only happens in the nucleus.

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2
Q

what is the primary transcript?

A

initial mRNA nucleotide sequence. also called the pre-mRNA or heterogenous nucleur RNA.

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3
Q

how is the primary transcript processed?

A

its processed in three ways,

1) addition of nucleotides,
2) deletion of nucleotides
3) modification of nitrogenous bases.

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4
Q

what is the job of the 5’ cap?

A

serves as an attachment site in protein synthesis and as protection against degradation

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5
Q

what is the 3’ end?

A

poly adenylated tail (poly A tail) - also protects against exonucleases.

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6
Q

before leaving the nucleus the primary transcript is cleaved into what?

A

introns and axons by snRNPs (snurps) - several snurps associate with proteins and they form a complex called spliceosome. inside the splicesome, introns are looped bringing the axons together. introns are excised and exons are cut together to form a single mRNA that will code for protein.

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7
Q

introns are degraded where?

A

nucleus.

exons exit the nucleus to be translated.

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8
Q

What is the reason that there are more proteins than genes?

A

due to diff splicing patterns of the same gene

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9
Q

translation is directed by what?

A

mRNA- carries the code form the nucleus to the cytosol in form of codons.

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10
Q

what about tRNA?

A

has a set of nucleotides that are complementary to the codon , called the anticodon. tRNA sequesters the aa that corresponds to the anticodon.

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11
Q

what about rRNA?

A

WITH protein makes up the ribosome, which provides a site for translation to take place.

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12
Q

what is the ribosome made up of?

A

small subunit and large subunit made from rRNA and many proteins.
prokaryotic ribosomes (30s and 50s, total 70s) are smaller then eukaryotic (40s and 60s= 80s)
ribosomes are made in the nucleolus- which is not in prokaryotes. the ribosome is assembled in the nucleolus , small and large subunit are exported separately to the cytoplasm.

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13
Q

what happens after post transcriptional processing in a eukaryote?

A

mRNA leaves the nucleus though the pores and enters the cytosol. with the help of initiation factors, the 5’ end attatches to the small subunit of ribosome. a tRNA with anticodon CAU, sequesters aa methionine and settles at the P site . this is a signal for the large subunit to join and form the initiation complex . this process is called initiation.

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14
Q

how does elongation of the polypeptide begin?

A

a tRNA with a corresponding aa attaches to the A site (aminoacyl site) at the expense of 2 GTPs . the carboxyl terminus of the methionine attaches the N terminus of the aa at the A site in a dehydration run catalyzed by peptide transferase (enz of the ribosome). then translocation occurs.

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15
Q

what happens in translocation?

A

tRNA with the methionine moves to the E site where it can exit the ribosome. a tRNA with a newly formed di peptide moves to the P site, clearing the A site for the next tRNA. translocation requires another GTP

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16
Q

How long is elongation continued?

A

until a stop codon reaches the P site. - that’s when termination occurs. when a stop codon reaches the A site, proteins known as the release factors will find to the A site - allowing for water molecule to add to the polypeptide chain. the poplypeptide is free from the tRNA and ribosome , the ribosome breaks up into subunits .

17
Q

what does the aa sequence determine?

A

the folding conformation ! even as the polypeptide is being translated , it begins to fold. the folding process is assisted by proteins called chaperones.

18
Q

What is added in post translational modifications?

A

sugars, lipids or phosphate groups may be added to amino acids.

19
Q

Proteins injected into the ER lumen are destined to become membrane bound proteins of ?

A

nucleus envelope, ER, golgi, lysosomes, plasma membrane or to be secreted from the cell.

20
Q

what is the signal peptide?

A

20 amino acid sequence , near the front of the polypeptide. its recognized by protein -RNA signal recognition particle, which will carry the entire ribosome complex to a receptor protein on the ER. there the protein grows across the mem, where its either released into the lumen or remains partially attached to the ER.

21
Q

Summary of translation

A

translation begins on a free floating ribosome. a signal peptide at the beg go the translated polypeptide may direct the ribosome to attach to the ER , in which case the polypeptide is injected into the lumen ( may be secreted from the cell via the golgi or may remain partially attatched to the membrane).

22
Q

The genetic code

A

mRNA nucleotides are strung togetherto form a genetic code which translates DNA nucleotide sequence into an aa sequence and ultimately to a protein.

23
Q

the genetic code is degenerative

A

more than 1 series of three nucleotides may code for any amino acid.

24
Q

unambiguous

A

any single series of three nucleotides will code for one and only one aa

25
Q

almost universal

A

nearly every living organism uses the same code.

26
Q

what is a codon?

A

three consecutive nucleotides on a strand of mRNA

27
Q

what are the stop codons?

A

UAA
UGA
UAG
These signal an end to protein synthesis .

28
Q

what is the start codon?

A

AUG, it also acts as a codon for the aa methionine.