Post Transcriptional processing and Translation Flashcards
post transcriptional processing of RNA occurs in …
both eukaryote and prokaryotic cells.
in prokaryotic cells, rRNA and tRNA go through it , but mRNA is directly translated into protein.
in eukaryotes each RNA type undergoes post transcriptional processing , which allows for further gene regulation
in eukaryotes , this only happens in the nucleus.
what is the primary transcript?
initial mRNA nucleotide sequence. also called the pre-mRNA or heterogenous nucleur RNA.
how is the primary transcript processed?
its processed in three ways,
1) addition of nucleotides,
2) deletion of nucleotides
3) modification of nitrogenous bases.
what is the job of the 5’ cap?
serves as an attachment site in protein synthesis and as protection against degradation
what is the 3’ end?
poly adenylated tail (poly A tail) - also protects against exonucleases.
before leaving the nucleus the primary transcript is cleaved into what?
introns and axons by snRNPs (snurps) - several snurps associate with proteins and they form a complex called spliceosome. inside the splicesome, introns are looped bringing the axons together. introns are excised and exons are cut together to form a single mRNA that will code for protein.
introns are degraded where?
nucleus.
exons exit the nucleus to be translated.
What is the reason that there are more proteins than genes?
due to diff splicing patterns of the same gene
translation is directed by what?
mRNA- carries the code form the nucleus to the cytosol in form of codons.
what about tRNA?
has a set of nucleotides that are complementary to the codon , called the anticodon. tRNA sequesters the aa that corresponds to the anticodon.
what about rRNA?
WITH protein makes up the ribosome, which provides a site for translation to take place.
what is the ribosome made up of?
small subunit and large subunit made from rRNA and many proteins.
prokaryotic ribosomes (30s and 50s, total 70s) are smaller then eukaryotic (40s and 60s= 80s)
ribosomes are made in the nucleolus- which is not in prokaryotes. the ribosome is assembled in the nucleolus , small and large subunit are exported separately to the cytoplasm.
what happens after post transcriptional processing in a eukaryote?
mRNA leaves the nucleus though the pores and enters the cytosol. with the help of initiation factors, the 5’ end attatches to the small subunit of ribosome. a tRNA with anticodon CAU, sequesters aa methionine and settles at the P site . this is a signal for the large subunit to join and form the initiation complex . this process is called initiation.
how does elongation of the polypeptide begin?
a tRNA with a corresponding aa attaches to the A site (aminoacyl site) at the expense of 2 GTPs . the carboxyl terminus of the methionine attaches the N terminus of the aa at the A site in a dehydration run catalyzed by peptide transferase (enz of the ribosome). then translocation occurs.
what happens in translocation?
tRNA with the methionine moves to the E site where it can exit the ribosome. a tRNA with a newly formed di peptide moves to the P site, clearing the A site for the next tRNA. translocation requires another GTP