POST LAB EXPERIMENT 11 Flashcards
• A pathologic process in which an extremely high fever is a characteristic manifestation.
febrile disease
• It is a group of microbial infections characterized by fever and production of antibodies known as
febrile agglutinins
febrile disease
Enumerate and describe other types of febrile diseases
Lyme Disease
Paratyphoid fever
Rocky Mountain spotted fever
Tularemia
Q Fever
Borrelia burgdorferi
febrile disease Lyme Disease
Lyme disease
febrile disease
MOT:
spread by the bite of infected ticks of the genus Ixodes.
- Erythema chronicum migrans
Stage 1: Early infection
- Spirochetes spread
hematogenously to body tissues by way of bloodstream and disease may take various forms.
Stage 2: Dissemination stage
- Symptoms include muscle and joint pain, fatigue, chills and fever, headache, swollen lymph nodes, secondary annular skin lesions.
Stage 2: Dissemination stage
- Commonly involves intermittent
episodes of joint pain which is
often misdiagnosed as arthritis.
Stage 3: Chronic disseminated
- Some common clinical manifestations of this stage can include meningitis, Bell’s palsy, cardiac involvement, migratory pain to joints, tendons, muscle and bone.
Stage 3: Chronic disseminated
The typical clinical presentation after inoculation of (?) through abrasions in the skin or by arthropod bites includes the development of a lesion at the site and progresses to an ulcer lymph node adjacent to the site of inoculation become enlarged and often necrotic.
F. tularensis
Once the organism enters the bloodstream, patients become systemically ill with high temperature, chills, headache, and generalized aching.
tularensis
Clinical manifestations of infection with (?) range from mild and self-limiting to fatal
- Symptoms occur approximately 7 days after a tick bite.
Rocky Mountain spotted fever
- A rash, which starts on the hands and feet and proceeds to the trunk, appears in 3 to 5 days after the beginning of other symptoms.
Rocky Mountain spotted fever
- The organism infects endothelial cells, causing an increase in vascular permeability and focal hemorrhages
Rocky Mountain spotted fever
Current serological assays for rickettsia antibodies are organism-specific and include
indirect fluorescent assays (IFA), microimmunofluorescent assays (micro-IF), immunoperoxidase assays (IPA), ELISA, and immunoblot assays (IBA)
require the whole bacterium as the reagent
- IFA and IPA
are currently considered the gold standard for detecting rickettsial antibodies
The IFA test and the micro-IF
OX-19 and OX-2: ?
Proteus vulgaris
OX-K: ?
Proteus mirabilis
(?) is useful for identifying several rickettsia diseases, such as murine typhus and Q fever.
The Weil-Felix test
Titers of 160 are usually considered significant.
The Weil-Felix test
which uses a light microscope instead of a fluorescent microscope to read the slides
IPA
Testing by both of these methods can detect significant titers of antibodies in Rocky Mountain spotted fever by the second week of infection.
IFA
IPA
appears in 60% to 80% of infected individuals 2-32 days after being bitten by infected tick.
Erythema chronicum migrans
use rickettsial antigens adsorbed onto a solid phase or nitrocellulose membrane.
- ELISA and IBA
They include glandular , ulceroglandular oculoglandular, oropharyngeal, systemic, and pneumonic forms.
F. tularensis
has a sensitivity and specificity similar to that of IFA
IPA
These include fever, severe headache, malaise, and myalgia, accompanied by nausea, vomiting, abdominal pain, and sometimes a cough.
Rocky Mountain spotted fever
(IFA)
indirect fluorescent assays
(micro-IF)
microimmunofluorescent assays
(IPA)
immunoperoxidase assays
(IBA)
immunoblot assays
