Photosynthesis Flashcards
What is the balanced formula for photosynthesis?
6CO2 + 6H20 -> 6O2 + C6H12O6
What are the two steps in photosynthesis?
Light-dependent reactions (thylakoid membrane, converts light into high energy molecules ATP and NADPH), Light-independent reactions (Calvin-cycle, stroma, uses high energy molecules ATP and NADPH to build carbohydrates (glucose))
Where are pigments found? What are accessory pigments? What types are there?
In clusters in the thylakoid membrane, accessory pigments absorb different wavelengths of light, more energy can be used overall, transfer light energy to chlorophyll a, Chlorophyll a (dark green, only pigment that passes energy to electrons) Chlorophyll b (light green) Xanthophyll (yellow) Carotenes (orange)
What are the different wavelengths of light?
380/400nm (purple), 750nm (red)
What are the most effective colours (for photosynthesis)? Least effective?
Blue and red, green (reflected)
What is chromatography? How do you calculate it?
Separating plant pigments based on how soluble they are in a solvent (high up = more soluble), Rf = pigment/solvent
What is the reduced form of NADP+?
NADPH
What does Photosystem II do?
Thylakoid membrane, photolysis splits H2O, solar energy used to pass energy to e to proton pump while O2 diffuses out leaf, H+ produced help form proton gradient
What does Photosystem I do?
Thylakoid membrane, after proton pump, e has low E, absorb photons for solar E re-energize e, NADP reduced by PSI giving it energy to make NADPH,
What is Chemiosmosis?
Thylakoid membrane, build up of H ions in thylakoid space (lumen), moving H+ along the hydrogen gradient to the stroma, H+ can only move through ATP synthase, which uses H+ energy go make ATP from ADP + P
What does the Calvin Cycle do?
6 cycles for 1 glucose, Uses NADPH, ATP and CO2 to make glucose, Carbon fixation (taken from “air” fixed into a molecule, RuBP (5 carbons) joins CO2 from atmosphere), Reduction (Hydrocarbon intermediate gets electrons from NADPH, NADPH oxidized to NADP+, ATP used to ADP, 2 Triose phosphate (G3P) created), Regeneration of RuBP (Triose phosphate combined to make RuBP using E from ATP), stroma
What do all photosynthetic organisms have in common?
They all contain chlorophyll a
What is produced as a result of photolysis?
Oxygen
What is photolysis?
PSII, light energy used to split water into O2 and H