Photosyntheis: Tlc Flashcards
What is the mobile phase
Where molecules can move (liquid solvent)
What is the stationary phase
Where molecules can’t move
Glass plate or Si gel
What does tlc stand for
Thin layer chromatography
What is the equation for rf
Rf = distance travelled by spot/distance travelled by solvent
What are the safety precautions
Lab coat
Eye protection
Gloves
Do in fume cupboard due to volatile liquids with toxic vapours
What do you add to the ground up leaves
Anhydrous sodium sulfate
Propanone
Petroleum ether
What is the in the top layer
Pigments + petroleum ether
What is added back to the top layer
Anhydrous sodium sulfate
What is in the solvent
Mixture of
Propanone
Cyclohexane
Petroleum ether
Why do different pigments separate
Move up the plate at different rates
Give method for tlc
- Grind up leaves with anhydrous sodium sulfate
- Add propanone
- Add petroleum ether and shake in test tube
- Separate top layer
- Add anhydrous sodium sulfate
- Draw pencil line at bottom of plate
- Make point if origin on pencil line
- Add solvent to bottom of plate
- Allow solvent to move up late then take out of solvent and mark solvent front in pencil
- Calculate rf
Repeat for shade tolèrent plant and shade intolerant plants
Why do shade tolèrent produce dark red and purple pigments
Protect chloroplasts momentarily from high light intensity
What are dark red and purple pigments called in shade tolèrent plants
Anthocyanins
How are shade tolèrent plants adapted
Have différèrent proportion of pigments
Adapted for low levels of light
