Calorimetry

Question 1

What reagent is used in calorimètry

Answer 1

Quantitative Benedicts reagent

Question 2

What happens to quantitative benedicts reagent

Answer 2

Blue -> coolourless

Question 3

What colour will Quant. Benedicts reagent go in high glucose conc

Answer 3

More blue lost
More colourless
Lower absorbance

Question 4

How do you do a serial solution with 5 tubes a dilution factor of 2 and initial conc of 4mM

Answer 4

1. Add 10cm of initial glucose to the first test tube
2. Add 5cm of distill water to next 4
3. Pipette 5 of glucose from first test tube into second
4. mix thoroughly
5. Repeat

Question 5

How to carry out quantative benedicts test

Answer 5

1. Add 2cm of quant. Ben to each test tube and to a control of pure water
2. Heat in water bath at the boil for 10 mins
3. Put in cuvette and calorimètre on the red filter
4. Measure absorbance

Question 6

What graph is plotted after a calorimètre

Answer 6

Calibration curve
X = glucose conc
Y = absorbance