Phosphoglycerate Kinase: Step 6 of Glycolysis

Question 1

What catalyzes the reaction of 1, 3-bisphosphoglycerate to 3-phosphoglycerate?

Answer 1

Phosphoglycerate kinase (PGK)

Question 2

Why is phosphoglycerate kinase (PGK) called a kinase even though it dephosphorylates 1, 3 BPG?

Answer 2

Because its reverse reaction phosphorylates 3-phosphoglycerate to make 1, 3 BPG.

Question 3

What cofactor is required for PGK to be a holoenzyme?

Answer 3

Mg2+

Question 4

What causes the two binding sites for ATP and 1, 3 bisphosphoglycerate on PGK (each on a separate domain) to swing together?

Answer 4

Binding of the substrates

Question 5

True or false: The reaction in glycolysis that is catalyzed by PGK is endergonic.

Answer 5

False

Question 6

What is a metabolic reaction that results in the formation of ATP or GTP by the direct transfer of a phosphoryl (PO3) group to ADP or GDP from another phosphorylated compound.

Answer 6

Substrate level phosphorylation

Question 7

What kind of reaction is the conversion of 1, 3-BPG to 3-PG?

Answer 7

Substrate level phosphorylation