Phosphofructokinase: Step 3 of Glycolysis Flashcards
Why reaction is catalyzed by phosphofructokinase?
The phosphorylation of fructose 6-kinase to form fructose-1, 6-bisphosphate.
Why is fructose-1,6-bisphosphate a “bis” rather than “bi” product?
Because the two phosphates are not directly attached to each other.
How is phosphofructokinase (PFK) similar to hexokinase?
It requires ATP and Mg2+ to phosphorylate its substrate.
What is the key regulatory enzyme for glycolysis?
Phosphofructokinase (PFK)
True or false: The reaction from fructose-6-phosphate (F6P) to fructose-1,6-bisphosphate (FBP) is reversible?
False
What molecule allosterically enhances the activity of PFK?
AMP
What allosterically inhibits PFK?
ATP and citrate
What enzyme catalyzes the reaction from F6P to FBP?
Phosphofructokinase
Why does AMP enhance the activity of PFK?
ATP breaks down into ADP and AMP, so high concentrations of AMP signal that ATP concentration is low.