PBL: Case 3 Part 2

Question 1

Gram stain: curved

Answer 1

Vibrio

Question 2

Gram stain: Gull-winged/curved/ s-shaped

Answer 2

Campylobacter

Question 3

Gram stain: dating lol?? Darting motility

Answer 3

C. jejuni

Question 4

Gram stain: wavy/ spiral (not in the stool)

Answer 4

Helicobacter

Question 5

What pathogens?

- mucous, blood, fever

Answer 5

**infections

Question 6

What pathogens?

- symptoms developing within minutes to few hours

Answer 6

intoxication

Question 7

What pathogens?

- Symptoms developing after 1 or more days

Answer 7

infections

Question 8

What pathogens?

- Rice water stools (severe dehydration, electrolyte imbalance)

Answer 8

V. cholera

Question 9

What pathogens?

- Pseudomembranous colitis (and megacolon)

Answer 9

C. difficile

Question 10

What pathogens?

- HUS

Answer 10

EHEC

Question 11

What pathogens?

- Guillain-Barre Syndrome

Answer 11

Campylocacter

Question 12

What pathogens?

- Carrier State (in gall bladder)

Answer 12

Salmonella typhi

Question 13

What pathogens?

- Peptic ulcers

Answer 13

Helicobacter pylori

Question 14

What pathogens?

- Appendicitis-like illness (specially in children

Answer 14

Y. enterolyica

Question 15

What pathogens?

- Sepsis

Answer 15

V. vulnificus

Question 16

Symptoms of acute diarrhea

Answer 16

Rapid onset, usually “enterotoxins-Mediated “= “intoxication”
- No fever, no blood, no white blood cells

Question 17

Set up for Campylobacter

Answer 17

Campy-Bap (microaeropilic at 42C)

Question 18

Media for Salmonella and reactions on that media

Answer 18

MAC: clear

HEC/XLD: black

Question 19

Media for Shigella and reactions on that media

Answer 19

MAC: clear
XLD: red/clear
HEK: green or clear

Question 20

Media for E. coli O157:H7 and reaction

Answer 20

SMAC - sorbitol negative (clear)

normal E. coli is pink on SMAC plate

Question 21

Media for Yersinia

Answer 21

YSA or CIN or MAC (incubate 33-35C …RT)

Question 22

Media for Aeromonas and Plesiomonas

• KIA?
• Oxidase?

Answer 22

Set up MAC (and others***)

Fermenters of glucose and oxidase positive

Question 23

Media for Vibrio?

• reaction with cholera and algenolyticus
• reaction for parahemolyticus and vulnificus

Answer 23

TCBS (high salt content)

• c and a = yellow (sucrose pos)
• p and v = green (sucrose neg)

Question 24

Media for C. difficile

Answer 24

CCFA (anaerobe incubation)

Question 25

Tests for Helicobacter

Answer 25

Breath Test (look for urea) 
CLO test on biopsy (rapid urease test)

Question 26

What would you test for Salmonella typhi first in the lab?

Answer 26

Take a blood first, early in the disease gets into the lymphatics to blood…blood then stool

Question 27

Transport medium for stool?

Answer 27

Cary Blair (especially for vibrio) 
Amies