PBL: Case 1 Part 1 Flashcards

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1
Q

Presence of bacteria in the blood

A

bacteremia

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2
Q

Bacteria passing through (dental, colonoscopy)

A

transient bacteremia

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3
Q

bacteria seeding from the infected site to another area intermittently (seeding blood) (same organism off and on)

A

intermittent bacteremia

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4
Q
  • Bacteria coming from an intravascular source all of the time (Endocarditis)
A

continous bacteremia

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5
Q

sepsis goin on inside a site such as an iv catheter, endocarditis

A

primary sepsis

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6
Q

sepsis going on in an external site such as strep from lungs, UTI, dental surgery, tooth accesses.

A

secondary sepsis

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7
Q

a contaminant in the blood

A

pseudobacteremia

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8
Q

bacteremia with organisms multiplying in blood and symptoms showing

A

septicemia

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9
Q

molds and yeast in the blood

A

fungemia

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10
Q

viruses in the blood

A

viremia

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11
Q

*Systemic inflammatory response syndrome (things must fall in a category?)

A

SIRS

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12
Q

inflammation of the cardiac lining

A

endocarditis

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13
Q

Sepsis with hypotension

A

septic shock

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14
Q

when the proteins that control blood clotting become over active and you get massive bleeding out

A

disseminated Intravascular coagulation (DIC)

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15
Q

blood culture system where the instrument checks for positive cultures 24-7

A

continuous monitoring blood culture systems

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16
Q

bacteria secreting _______ are notorious for septic shock

A

endotoxins

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17
Q

common symptoms and abnormal lab tests that would be associated with possible sepsis

A
  • fever (i or d)
  • shaking
  • chills
  • tachypnea
  • prostration (laying down)
  • blood pressure (i or d)
  • DIC
  • WBC count (i or d)
  • lactic acidosis
  • C-RP (i)
  • procalcitonin (indicator of inflammation on high levels indicative of septicemia)
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18
Q

Proper method of collecting a blood specimen for bacteriologic culture (5 steps)

A
  1. alcohol
  2. iodine based
  3. make circles going out
  4. draw opposite arm that has iv
  5. draw below iv if have to draw from arm with iv
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19
Q

correlate the number, timing and volume of specimens with optimal recovery of organisms for meaningful clinical use

A

Number: one set = one aerobic bottle and one anaerobic bottle; no more then 3 sets in 24 hours
Volume: 8-10 for adults and 1-3 for babies;
Timing: take blood before antibiotics or any therapy and right before a fever

20
Q

blood to medium ratio when collecting a blood

A

1:5- 1:10

21
Q

IV catheter related

A

Skin flora:

  • alpha/gama strep
  • CoNS
  • Propionibacterium ances
  • staphylococcus sacchrolyticus
  • Corynebacterium spp.
22
Q

UTI

A
  • E. coli (coming from GI)
23
Q

Subacute endocarditis

A
  • CoNS
  • Veridans strep
  • (s. aureus)
24
Q

acute endocarditis

A
  • staph aureus
  • enterococcus
  • strep pneumo
25
Q

Gunshot to the abdomen

A

GI flora:

  • E. coli
  • Other enterobacteriaceae
  • Enterococcus
  • anaerobes (B. frag, Clostridium, Bifidobacterium, anaerobic coccci, Fusobacterium)
26
Q

Oral surgery

A

Mouth Flora:

  • alpha and gamma streptococci
  • Corynebacterium
  • CoNS
  • non-pathogenic Neisseria app.
  • Prevotella melaninogenica
  • Porphyromonas spp.
  • Prevotella spp.
27
Q

Most common contaminants found in the blood

A

Skin flora:

  • alpha/gama strep
  • CoNS
  • Propionibacterium ances
  • staphylococcus sacchrolyticus
  • Corynebacterium spp.
28
Q

Immunocomporomised

A

anything really; fungi

29
Q

Preexisting pneumonia is the most common cause of septicemia. This pneumonia is caused by….

A

S. pneumo

Klebs. pneumo

30
Q

BACTEC system

A

measuring CO2 by fluorescences

31
Q

BacT/Allert system

A

measuring CO2 by pH change

32
Q

SeptiCheck

A

agar paddle and you inoculate it

33
Q

Lysis Centrifugation

A

(saphonin lysis white blood cells) this is for: intracellular pathogens and fungi, yeasts and molds

34
Q

What is the purpose of sodium polyanetholsulfonate (SPS)

A

anticoagulant

35
Q

What is the purpose of resins (ARD antimicrobial removal devices) in blood culture systems

A

neutralizes antibiotics

36
Q

Blood culture procedure for possible brucellosis

A

Castanata bottle/biphasic bottle: used when takes a long time to grow brucellosis (4 weeks)

37
Q

What approaches could be used to determine whether an isolate is a skin contaminant or a pathogen

A

Collect multiple specimens (3 sets a day max); Skin contaminant: 1 out of all the bottles positive Pathogen: all positive more likely to be a pathogen

38
Q

What one gram POSITIVE organism may be observed on a gram stained smear from a blood culture bottle but would NOT grow on SBA and, with some organisms, not on CHCO

A
  • Anaerobes

- Streptococcus abiotrophia (nutrient varient)

39
Q

What one gram NEGATIVE organism may be observed on a gram stained smear from a blood culture bottle but would NOT grow on SBA and, with some organisms, not on CHCO

A
  • Haemophilus (only grows on chocolate)
  • Campylobacter (charcoal containing media, not blood OR chocolate)
  • anaerobes
40
Q

What media should be set up on a positive blood culture, based on results of a positive smear

A
  • blood
  • choc
  • anaerobe blood
  • do preliminatry susceptibilites, - - GLC for anaerobes
41
Q

What test should you set up if you think you have a strep?

- of these which can you set up directly from the blood?

A
  1. Catalase
  2. NaCl
  3. PYR
  4. Bile Esculin
  5. CAMP
  6. Optochin (bile solubility)
  7. Bacitracin
    Set up from blood: Bile Esculin and optochin
42
Q

If you have a gram positive cocci what should you do first?

A

Gram stain, plate it out, and do optochin right away!

43
Q

What would you do if your gram stain was clumped (staph)

A

Do a coag right away (in 4 hours) FROM BLOOD

44
Q

What would you do if your gram stain was a GNR

A

Vitek or KIA FROM BLOOD

45
Q

We keep blood bottles for how many days?

A

5 days

46
Q

What is the significance if positive on the first day v.s. the fifth day?

A

First day: more likely to be significant

Fifth day: more likely to be a contaminant

47
Q

What is the best way to do a gram stain on a blood?

A

Methanol Fixation because it preserves red cells the best