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Microbiology Module 2 > Antimicrobial Susceptibility Testing PART 2 > Flashcards

Antimicrobial Susceptibility Testing PART 2 Flashcards

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Biology 101 flashcards
1
Q

Standardizing susceptibility testing:

- growth medium

A

Mueller-Hinton Agar or broth

- deph 3-5mm

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2
Q

Standardizing susceptibility testing:

- pH

A

7.2 to 7.4

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3
Q

Standardizing susceptibility testing:

- cation concentration

A

Magnesium, calcium, sodium chloride

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4
Q

Standardizing susceptibility testing:

- incubation conditions

A

35C, ambient air, duration varies

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5
Q

Standardizing susceptibility testing:

- inoculum density

A

*If not enough organism then not enough enzyme which could lead to a false susceptibility

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6
Q

Disk Diffusion

- Inoculum prep

A

Three to five colonies made into a McFarland standard (Directly: put colonies into broth and measure turbidity, or Indirectly: touch colonies and let it grow in incubator)

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7
Q

Disk Diffusion

- McFarland Standard

A

Use barium sulfate to make a 1.5x10^8 CFU/ml standard which is a 0.5 McFarland

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8
Q

Disk Diffusion

- Inoculation of agar

A

Within 15 minutes of prep, streak plates in 3 planes so that there is a lawn of confluent growth

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9
Q

Disk Diffusion

- Application of disks

A

Within 15 minutes of inoculation, never relocate a disk

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10
Q

Disk Diffusion

- Inubation

A
  • invert plates
  • stack no more than 5 high
  • 16-18 hours
  • 35C ambient air
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11
Q

Disk Diffusion

- Reading results

A
  • Reflected vs transmitted

- read circular zone side in mm; if it is resistant zone size is still 6 mm!!! Never zero!

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12
Q

Disk Diffusion

  • Interpretation of Results
  • Quantitative or Qualitative
A

Qualitative results

- use CLSI charts to state S, I, or R

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13
Q

E-test

- Zone shape

A

elliptical

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14
Q

E-test

- MIC

A

where growth intersects strip

  • no zone: > highest concentration
  • Zone below strip: < lowest concentration
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15
Q

E-test

- Quantitative or Qualitative

A

Quantitative

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16
Q

Regression Curve

  • X axis
  • Y axis
A 
Y-axis: MIC (microgram/ml) 
X-axis: Zone size 
- these two are linearly inversely proportional
- as Zone size increases, MIC decreases 
Top Left = resistant
Bottom Right = susceptible

Microbiology Module 2 (19 decks)

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