part6 Flashcards
Which protein kinase is directly inhibited by Rapamycin?
mTOR
What is the main application of yeast two-hybrid screening?
Protein protein interactions
Name two signalling molecules which are negative regulators of cell size and growth.
Cdr2
A mannose 6-phosphate signal added to a soluble protein in the Golgi apparatus directs it to which organelle?
lysosome
The vesicles that carry cargo from the cis Golgi to the ER have which type of coat?
Cop1
Which one of these amino acid sequences is a putative endocytosis signal:-
a) –KKXX, b) –KDEL, c) –NPXY-, d) –NXS-?
npxy
“Shibire” flies are paralysed at the non-permissive temperature because they have a temperature-sensitive mutation in which GTPase?
dyamin
Name the intermediate filament protein that is found in hair.
Keratin
Which adhesion protein makes a homophilic dimer between two neighbouring cells?
Cadherin
Name the actin binding protein which catalyses actin filament depolymerization.
ADF
What is the homologue of actin found in bacteria?
-
mReb
What is the function of the FtsZ protein in bacteria?
-
During cell division, FtsZ is the first protein to move to the division site, and is essential for recruiting other proteins that produce a new cell wall between the dividing cells. FtsZ’s role in cell division is analogous to that of actin in eukaryotic cell division, but, unlike the actin-myosin ring in eukaryotes, FtsZ has no known motor protein associated with it.
This is a prokaryotic homologue to the eukaryotic protein tubulin.
FtsZ has been named after “Filamenting temperature-sensitive mutant Z”
The bacterial flagellar motor has parts that rotate against a static part in the cell membrane. What is the name give to the static part of this motor?
-
MS ring???
A protein containing a basic N-terminal amphipathic alpha helix is most likely to be targeted to which one of the following organelles: a) peroxisomes, b) mitochondria, c) endoplasmic reticulum, d) nuclei?
mitochondria
Which one of the following receptors does not interact with part of the polypeptide chain: a) TOM20, b) Pex5p, c) mannose-6-phosphate receptor, d) importin alpha?
Importin alpha (right answer as protein is pre-folded)
Name an autofluorescent protein extracted from jellyfish which has an excitation peak at 396nm and an emission peak at 509 nm.
GFP
To the nearest thousand, how many genes does Escherichia coli have?
5000
What is the main enzyme in peroxisomes that degrades H2O2?
catalase
The spontaneous formation of lipid membranes is encouraged by the reduction in which thermodynamic property?
repulsion
Name a phopspholipid not usually found on the outer surface of healthy cells but which appears during apoptosis.
PS
Name two protein secondary structural elements that can span cell membranes.
Alpha helices, beta barrels
Name one member of the cys-loop family of ionotropic receptors.
5HT (?)
Which protein complex binds sister chromatids together following DNA replication?
cohesins
Name the process whereby repeated rounds of DNA replication take place in the absence of mitosis.
Endoreduplication
