part5

Question 1

Which one of the following organelles receives most of its protein contents directly from the endoplasmic reticulum:- a) peroxisomes, b) Golgi apparatus,
c) mitochondria, d) nucleus?

Answer 1

Golgi

Question 2

What does FRAP stand for?

Answer 2

Fluorescent recovery after photobleaching

Question 3

About how many different types of protein make up the nuclear pore in Saccharomyces cerevisiae:- a) 3, b) 30, c) 300, d) 3000?

Answer 3

30

Question 4

Which is the only one of the following statements that could possible be true as a description of a fluorochrome:- a) it absorbs blue light and emits yellow;

b) it absorbs yellow light and emits blue;
c) it absorbs red light and emits yellow;
d) it absorbs yellow light and emits green?

Answer 4

a

Question 5

Cultured CHO cells are commonly used in cell biology experiments. What does CHO stand for?

Answer 5

Chinese hamster ovaries

Question 6

Which protein removes the inhibitory phosphates from CDKs?

Answer 6

Wee1

Question 7

Name two ubiquitin ligases important in cell-cycle regulation.

Answer 7

SCF, APC

Question 8

Name two of the inhibitors of CDK4/6.

Answer 8

P15, p16

Question 9

Which one of the following is NOT a type of white blood cell:-
a) granulocyte, b) erythrocyte, c) lymphocyte, d) basophil?

Answer 9

b

Question 10

What differentiated neural cell type in the central nervous system produces myelin?

Answer 10

oligodendrocyte

Question 11

What one of the following is the function of transcription factors:- a) to block growth factor signalling, b) to activate but not repress gene transcription, c) to activate and/or repress gene transcription, d) to transcribe genes?

Answer 11

c

Question 12

What biological process eliminates excess cells during development?

Answer 12

apoptosis

Question 13

Which one of the following toxins does NOT target a cytoskeleton protein:-
a) phalloidin, b) latrunculin, c) ricin, d) colchicines?

Answer 13

c

Question 14

What apparatus in the cell drives segregation of chromosomes at mitosis?

Answer 14

MT

Question 15

The vesicles that carry cargo from the endoplasmic reticulum to the cis-Golgi are coated with which type of coat?

Answer 15

COPII

Question 16

What does FRET stand for?

Answer 16

Flourescent resonance energy transfer

Question 17

In which one of the following organelles does protein import occur mainly co-translationally:- a) mitochondria, b) nucleus, c) endoplasmic reticulum, d) peroxisomes?

Answer 17

ER

Question 18

What is the term used to describe the highly concentrated solution of proteins and other macromolecules inside cells?

Answer 18

Cytoplasm

Question 19

Name two ubiquitin ligases important in cell-cycle regulation.

Answer 19

APC, SCF

Question 20

What gives the rough endoplasmic reticulum its characteristic “rough” appearance on electron micrographs?

Answer 20

Ribosomes

Question 21

Which ATPase complex is thought to dissociate cis-SNARE complexes after membrane fusion?

Answer 21

NSF

Question 22

Which one is of these is a phospholipid:- a) phosphatidylcholine, b) phosphatidylguanosine, c) diacylglycerol, d) phosphatidylinosine?

Answer 22

a

Question 23

What is the major mitochondrial protein that initiates apoptotic cell death?

Answer 23

cytochrome c

Question 24

Rank the following commonly used model organisms in terms of their genome size in MB (largest first):- a) Dictyostelium discoideum, b) Xenopus laevis, c) Escherichia coli, d) Arabidopsis thaliana.

Answer 24

D, b, a, c

Question 25

What is dynein?

Answer 25

a protein which binds to MT, motor protein

Question 26

Name the protease that cleaves securin to trigger anaphase.

Answer 26

APC

Question 27

A membrane protein with the C-terminal signal KKXX would be found in which one of the following organelles:-organelle? a) endoplasmic reticulum b) endosomes c) lysosomes d) mitochondria?

Answer 27

ER

Question 28

Name a normal constituent of membranes that increases membrane fluidity as its concentration is increased.

Answer 28

cholesterol

Question 29

What does PCR stand for?

Answer 29

polymerase chain reaction

Question 30

SDS-PAGE separates globular proteins and polypeptides on the basis of which property?

Answer 30

molecular weight