part1 Flashcards
Which one of the following organelles contains a transcription machinery –
a) lysosome, b) peroxisome, c) chloroplast, d) endoplasmic reticulum?
Chloroplast
If a polypeptide possesses an N-terminal positively-charged amphipathic alpha-helix, which organelle is it most likely to be targeted to?
Mitochondria
Which one of the following is the main function of the stacking gel in SDS-PAGE:- a) to separate proteins, b) to concentrate proteins, c) to diffuse proteins, or d) to reduce proteins?
To Separate Proteins
If fluorochrome X absorbs light of wavelength 480 nm, which one of the following statements cannot be true:- a) fluorochrome X emits blue light,
b) fluorochrome X emits green light, c) fluorochrome X emits yellow light,
d) fluorochrome X emits red light?
Flurochrome emits Blue light
Which two of the following disrupt microtubules - a) cytochalasin, b) taxol,
c) phalloidin, d) nocodazole?
Nocodazole and Taxol
What microtubule motor protein is found in cilia?
dynein
Which two of the following are found in the basal lamina - a) laminin,
b) collagen III, c) fibronectin, d) perlecan?
laminin and fibronectin
Which proteins bind sister chromatids together?
Cohesins
Name two ubiquitin ligases that regulate the cell-cycle.
APC/C and SCF
Name the phosphatase that activates M-CDKs driving entry into mitosis.
Cdc25
Which one of the following ionotropic receptors is formed of four individual polypeptide chains forming a tetramer – a) nicotinic acetylcholine receptor,
b) glutamate receptor, c) GABA receptor, or d) glycine receptor?
Glutamate
Measuring the reversal voltage of current through an ion channel can allow deductions to be made concerning which one its properties:- a) selectivity,
b) gating, c) inactivation, or d) subconductance?
selectivity
Which two of the following proteins are both enzymes and carriers:- a) Band 3,
b) sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA), c) glucose transporter, d) electron transport chain, or e) sodium/calcium exchanger?
SERCA and electron transport chain
What it the terminal compartment of the endocytic pathway?
lysosome
How many subunits are there in a prototype clathrin adaptor complex
(e.g. AP-1/2)?
four
Define the word “transdifferentiation” in one sentence.
One mature somatic stem cell transforms into another mature somatic stem cell without undergoing an intermittent pluripotent state or progenitor stem cell type
At what stage of embryonic development are ES cells derived?
blastocyst
What is an adult muscle stem cell called?
somatic stem cell
Name two proteins that interact with Rb protein.
Cyclin D1, Insulin
Apart from motor neurons, which one of the following is the other major cell type that is generated in the pMN domain in the spinal cord:- a) astrocytes,
b) inter neurons, c) oligodendrocytes, or d) macrophages?
oligodendrocytes
Which one of the following is the major function of oligodendrocytes:-
a) specification of cell fates, b) removal of excess neurotransmitters at synapses, c) myelination of axons, or d) innervation of target tissues?
myelination of axons
Which class of proteases is activated during apoptosis?
caspases
Is the following statement true or false: “inflammation is absent during apoptosis”?
true
How thick is a “lipid bilayer” – a) 6 nm, b) 60 nm, c) 600 nm, or d) 6µm?
6nm
