part4

Question 1

Giant polytene chromosomes are found in the salivary gland of which model organism?

Answer 1

Drosophila

Question 2

CHO, HeLa, MDCK and 3T3 are all types of what?

Answer 2

Mammalian cell culture

Question 3

The jellyfish Aequorea Victoria is the source of which protein commonly used in cell biology experiments?

Answer 3

GFP

Question 4

For a typical immuno-electron microscopy experiment, place the following processes in the correct temporal order: a) immunolabelling, b) embedding, c) fixation, d) sectioning.

Answer 4

d, c, b, a

Question 5

To which organelle is a protein containing the internal sequence PKKKRKV most likely to be targeted to?

Answer 5

Nucleus

Question 6

Approximately how many different types of protein are found in the nuclear pore: a) 3, b) 30, c) 300, d) 3000?

Answer 6

b

Question 7

Which organelles fill up with undegraded macromolecules in patients with GM2 gangliosidosis?

Answer 7

lysosomes

Question 8

In which part of the mitochondrion is the enzyme citrate synthase found: a) outer membrane, b) intermembrane space, c) inner membrane, d) matrix.

Answer 8

matrix

Question 9

The nematode C. elegans is a commonly used model organism. What does the C stand for? (Write the answer legibly in capital letters).

Answer 9

caenorhabitatis

Question 10

How many mitotic divisions occur in meiosis?

Answer 10

2

Question 11

Name the two CDKs that bind to cyclin D in mammalian cells.

Answer 11

cdc 4, 6

Question 12

Name one ubiquitin ligase important in the cell-cycle.

Answer 12

APC, SCF

Question 13

What is the thickness of a typical cellular membrane? (do not forget the units!)

Answer 13

6nm

Question 14

Name one lipid or class of lipids that is entirely asymmetrically sorted to one or other leaflet of a normal healthy plasma membrane.

Answer 14

Phosphotidylserine

Question 15

What is the fate of lysosomal enzymes in cells from patients with mucolipidosis type-II (I-cell disease)?

Answer 15

get secreted

Question 16

In which one of the following organelles would a soluble protein with the C-terminal sequence KDEL be found : a) lysosomes b) mitochondria c) endosomes d) endoplasmic reticulum?

Answer 16

ER

Question 17

The Drosophila Shibire mutant has a temperature-sensitive mutation in which protein?

Answer 17

dynamin

Question 18

In which direction does dynein move on microtubules?

Answer 18

• end

Question 19

In which direction does myosin II move on actin filaments?

Answer 19

+ end

Question 20

Which one of the following structures in cells are NOT composed of dynamic cytoskeleton polymers: a), mitotic spindles, b) flagella c) lamellipodia, d) muscle fibres?

Answer 20

flagella

Question 21

The selectivity filter of the voltage gated calcium channel is generated by the side chains of which amino acid?

Answer 21

glutamate

Question 22

Measuring the reversal voltage of current through a single ion channel can allow deductions to be made concerning which one of the following channel properties: a) selectivity, b) gating, c) inactivation, d) subconductance behaviour?

Answer 22

a

Question 23

What is the structure that bacteria use to swim?

Answer 23

flagella

Question 24

What is another name for bacterial lipopolysaccharide or LPS?

Answer 24

endotoxin ???

Question 25

List the four different shapes that different bacterial cells can take.

Answer 25

``` spherical (cocci), rod (bacilli), spiral (spirilla), comma (vibrios) or corkscrew (spirochaetes) ```

Question 26

Which two of the following cell types are found in the nervous system: a) granulocyte/macrophage precursor cells, b) myloid precursor cells, c) oligodendrocyte precursor cells, d) astrocytes?

Answer 26

C, d

Question 27

The ced genes are important for controlling which two of the following: a) apoptosis, b) differentiation, c) final cell numbers, d) necrosis?

Answer 27

a, c

Question 28

Which two of the following are properties of transcription factors: a) self renewal, b) bind to DNA, c) bind to cell surface receptors, d) control gene expression?

Answer 28

b, d

Question 29

Name two tumour suppressors that are involved in the regulation of cell growth.

Answer 29

p53, APC

Question 30

Which two of the following are commonly used techniques for the visualization of proteins in SDS-PAGE gels: a) Coomassie Blue staining, b) gold staining, c) silver staining, d) Texas Red staining?

Answer 30

a, c