part3 Flashcards
With which one of the following cellular membranes is the nuclear envelope continuous:- a) ER, b) plasma membrane, c) peroxisomal membrane, d) nucleolar membrane?
Endoplasmic Reticulum
In which mitochondrial sub-compartment are enzymes of the Kreb’s (citric acid) cycle found?
Mitochondrial Matrix
The ribosomes are the main site of which one of the following cellular processes:- a) transcription, b) glycosylation, c) translation, d) phosphorylation?
Translation
Which one of the following is the mitotic spindle a specialized form of:-
a) microtubule, b) microfilament, c) intermediate filament, d) nuclear envelope?
Microtubule
Which one of the following does not receive any significant proteinaceaous contributions from the ER:- a) Golgi apparatus, b) lysosomes, c) mitochondria, d) plasma membrane?
Mitochondria
Which one of the following receptors is found in the mitochondrial outer membrane:- a) TOM20, b) mannose-6-phosphate receptor, c) Pex5p, d) importin-alpha?
TOM20
In immunofluorescence microscopy, what does the abbreviation FITC stand for?
Fluorescin Isothiocyanate
Sedimentation rate density-gradient centrifugation separates organelles on the basis of which property?
Size
Name two proteins that bind to b-catenin.
GSK, APC
Which two of the following intermediate filament proteins can form heteropolymers:- a) keratins, b) lamins, c) vimentin, d) neurofilaments?
Keratins
The transferrin receptor is involved in the uptake of which two of the following:-¬ a) cholesterol, b) antibodies, c) iron, d) some viruses?
Iron
Name two adaptor proteins involved in clathrin-mediated endocytosis.
AP1, AP2,
What is the most common post-translational modification found in signal transduction?
Phosphorylation
Western blotting is used to detect which one of the following:- a) DNA, b) RNA, c) proteins, d) carbohydrates?
Protein
Which channel property can be deduced from the value of the reversal voltage?
Specificity
Which one of the following most accurately describes the quaternary structure of potassium channels:- a) monomeric, b) dimeric, c) tetrameric, d) octameric?
Tetrameric
Which one of the following most closely approximates to the thickness of a phospholipid bilayer of the sort found in eukaryotic cells:- a) 60 nm, b) 6 nm,
c) 6 Å, d) 600 Å?
6nm
What change in a membrane’s properties would you expect to see upon the incorporation of cholesterol or phospholipids containing fatty acyl chains with cis-double bonds?
Greater Fluidity
Name one class of enzymes that hydrolyse phosphodiester bonds in phospholipids.
phospholipases
Name the two ubiquitin ligases important in regulating the cell cycle.
APC/C and SCF
Name the two cyclins that complex with CDK1 in mammalian cells.
Cyclin A and Cyclin B
Which protein cleaves the cohesins that bind sister chromosomes together?
Separase
Which class of proteases is activated during apoptosis?.
Caspase
Name two proteins that are released from the mitochondria during apoptosis.
Cytochrome c, AIF (apoptotic inducing factor)
