Papovaviridae

Question 1

• Naked icosahedral capsid containing circular double stranded DNA
- replication: __
- MOT: direct contact, resp droppets, bodily fluids
- dse in human and animals (warts, cx, neuro disorder)

Answer 1

Papovaviridae
Pa-Papilloma
Po-Polyoma
Va-Vacuolating
- nucleus

Question 2

• 2 subfamilies of Papovaviridae

Answer 2

1 Papillomaviridae-Warts, condylomas, papillomas, cervical cancer
2 Polyomaviridae-BK Virus, JC virus, Merkel Cell Polyomavirus, Trichodysplasia spinulosa Polyomavirus

Question 3

• ~55nm
• Can cause infections in humans, dogs, cattle, monkeys and many other species
• -causes __
• Viral tropism-___
• Divided into more than __ serotypes
- ___ stds: __ oncogenic

Answer 3

Papillomavirus
- warts
- cutaneous/mucosal tissue
- 200
-40
12

Question 4

Non malignant lesion

Answer 4

Common warts 1,2,4 Hpv genotype
Flat warts 3 hpv genotype
Genital warts 6, 11, hpv genotype
Laryngeal Papilloma 6, 11 hpv genotype

Question 5

Non malignant lesion

Answer 5

Common warts 1,2,4 Hpv genotype
Flat warts 3 hpv genotype
Genital warts 6, 11, hpv genotype
Laryngeal Papilloma 6, 11 hpv genotype

Question 6

( asso w/ cancer) need for surgical removal-> damge to __ and __

Answer 6

hpv 6 and 11
- vocal cords and resp obstruction

Question 7

Premalignant lesions

Answer 7

Epidermodysplasia verruciformis 2,3 and others

Question 8

Malignant lesions

Answer 8

Cervical cancer 16,18,31, 33,35 and others

Question 9

Envelope proteins __ and __ are transforming proteins associated with
initiating cancer

Answer 9

E6 and E7

Question 10

HPV high risk genotypes cause __,___,__,__,__,__ .

Answer 10

cervical, vulvar, vaginal, squamous cell oropharynx, anal and penile cancer

Question 11

95% of all cervical cancers are HPV. 70% of which is caused by HPV __ and ___

Answer 11

16 and 18

Question 12

Epidemiology
• MOT: Sexual, Vertical, Direct contact with infected material
• Incubation period: genital warts- ___ , cancer-___ from the acquisition of infection

Answer 12

Papillomaviruses
- 3 months after exposure
- several years

Question 13

Skin
1
2
3

Answer 13

1. Common Warts (Verruca vulgaris)-HPV 1, 2, 4 (common where the skin is broken, back of hand, biting of finger nail/ picking hand nail)
2. Flat Warts (Verrucae plana) HPV 3 ( smoother and smaller than other warts)
3. Epidermodysplasia verruciformis (suscep to cutaneous hpv: life long erruption in assymp lesion)

Question 14

Mucus Membrane
1
2

Answer 14

1. Genital Warts-HPV 6, 11
2. Laryngeal Warts-HPV 6, 11

Question 15

Carcinoma
1
2

Answer 15

1. Cervical cancer-HPV 16, 18, 31, 33, 35
2. Other cancers-squamous cell carcinoma (penis, vulva, vagina,
   laryngeal carcinoma)

Question 16

Papillomaviruses
• Diagnosis: __,__,___,__
• Treatment/Prevention:
• Self-limiting and normally recede in __
• Ablative treatments with __ or __

Answer 16

Histopathologic or cytologic examination of cutaneous biopsy or cells, DNA probe assays, NAAT
- 6-12 months
- podophyllin or liquid nitrogen

Question 17

Papilloma Vaccines: prevents the most carcinogenic type (hpv 16 and 18)
- cervarix- (hpc 16&18)
- gadrasil all cervarix
- gadrasil 9 all cerv and gadrasil

Answer 17

Cervarix (HPV 16 and 18 and cross protection w/ hpv 31,33,35)
Gardasil (HPV 16 and 18 and cross protection w/ hpv 31,33,35) and ( HPV 6 and 11)
Gardasil-9: all cervarix and gardasil plus (HPV 31, 33, 45, 52, 58)

Question 18

• ~40-45nm icosahedral, naked, circular double-stranded DNA
1
2
3
4
• Believed to be transmitted through respiratory or oral secretions
• Infections of these viruses usually occur during childhood and appears to have little clinical significance.
• Latency in __ and ___ can result symptomatic reactivation
• MOT: Respiratory droplets

Answer 18

Polyomavirus
- John Cunningham (JC), BK Virus, Merkel Cell Polyomavirus,Trichodysplasia spinulosa polyomavirus
- kidney and B lymphocytes

Question 19

• Common infection of childhood
• Had been isolated in urine from children with ___ and evidence of
infection has been demonstrated in children with ___
• Reactivation in renal transplant patients- ____,
bone marrow transplant patients-___
• Treatment: ___
• Diagnosis:
1
2

Answer 19

BK Virus
- cystitis
- acute respiratory illness
- urethral structures
- hemorrhagic cystitis
- Cidofovir
- PCR (blood and urine), Electron Microscopy of urinary sediments

Question 20

• Clinical importance is in patients who are immunosuppressed due to__ in whom the virus reactivates to cause ___
• Diagnosis: __,___,___

Answer 20

JC Virus
- T-cell dysfunction
- progressive multifocal leukoencephalopathy (PML)
- PCR (Brain biopsy or CSF), Serological Assays, Histological examinations

Question 21

• Clinical importance is in patients who are immunosuppressed due to__ in whom the virus reactivates to cause ___
• Diagnosis: __,___,___

Answer 21

JC Virus
- T-cell dysfunction
- progressive multifocal leukoencephalopathy (PML)
- PCR (Brain biopsy or CSF), Serological Assays, Histological examinations

Question 22

integrates its DNA into the genome of __
• Diagnosis: __, __
Treatment:__,___

Answer 22

Merkel Cell Polyomavirus
- Merkel Cells
- Tumor biopsy, PCR
- Surgery, Immunotherapy

Question 23

• Growth of abnormal hair-like structures in the skin
• Clinical presentation: red scaly patches on skin, itching or discomfort, hair like growths
• Diagnosis: __
Treatment: __,__,__

Answer 23

Trichodysplasia Spinulosa Polyomavirus
- Skin biopsy |
- Cidofovir cream, Corticosteriods,
Laser therapy