Papovaviridae Flashcards
• Naked icosahedral capsid containing circular double stranded DNA
- replication: __
- MOT: direct contact, resp droppets, bodily fluids
- dse in human and animals (warts, cx, neuro disorder)
Papovaviridae
Pa-Papilloma
Po-Polyoma
Va-Vacuolating
- nucleus
• 2 subfamilies of Papovaviridae
1 Papillomaviridae-Warts, condylomas, papillomas, cervical cancer
2 Polyomaviridae-BK Virus, JC virus, Merkel Cell Polyomavirus, Trichodysplasia spinulosa Polyomavirus
• ~55nm
• Can cause infections in humans, dogs, cattle, monkeys and many other species
• -causes __
• Viral tropism-___
• Divided into more than __ serotypes
- ___ stds: __ oncogenic
Papillomavirus
- warts
- cutaneous/mucosal tissue
- 200
-40
12
Non malignant lesion
Common warts 1,2,4 Hpv genotype
Flat warts 3 hpv genotype
Genital warts 6, 11, hpv genotype
Laryngeal Papilloma 6, 11 hpv genotype
Non malignant lesion
Common warts 1,2,4 Hpv genotype
Flat warts 3 hpv genotype
Genital warts 6, 11, hpv genotype
Laryngeal Papilloma 6, 11 hpv genotype
( asso w/ cancer) need for surgical removal-> damge to __ and __
hpv 6 and 11
- vocal cords and resp obstruction
Premalignant lesions
Epidermodysplasia verruciformis 2,3 and others
Malignant lesions
Cervical cancer 16,18,31, 33,35 and others
Envelope proteins __ and __ are transforming proteins associated with
initiating cancer
E6 and E7
HPV high risk genotypes cause __,___,__,__,__,__ .
cervical, vulvar, vaginal, squamous cell oropharynx, anal and penile cancer
95% of all cervical cancers are HPV. 70% of which is caused by HPV __ and ___
16 and 18
Epidemiology
• MOT: Sexual, Vertical, Direct contact with infected material
• Incubation period: genital warts- ___ , cancer-___ from the acquisition of infection
Papillomaviruses
- 3 months after exposure
- several years
Skin
1
2
3
- Common Warts (Verruca vulgaris)-HPV 1, 2, 4 (common where the skin is broken, back of hand, biting of finger nail/ picking hand nail)
- Flat Warts (Verrucae plana) HPV 3 ( smoother and smaller than other warts)
- Epidermodysplasia verruciformis (suscep to cutaneous hpv: life long erruption in assymp lesion)
Mucus Membrane
1
2
- Genital Warts-HPV 6, 11
- Laryngeal Warts-HPV 6, 11
Carcinoma
1
2
- Cervical cancer-HPV 16, 18, 31, 33, 35
- Other cancers-squamous cell carcinoma (penis, vulva, vagina,
laryngeal carcinoma)
Papillomaviruses
• Diagnosis: __,__,___,__
• Treatment/Prevention:
• Self-limiting and normally recede in __
• Ablative treatments with __ or __
Histopathologic or cytologic examination of cutaneous biopsy or cells, DNA probe assays, NAAT
- 6-12 months
- podophyllin or liquid nitrogen
Papilloma Vaccines: prevents the most carcinogenic type (hpv 16 and 18)
- cervarix- (hpc 16&18)
- gadrasil all cervarix
- gadrasil 9 all cerv and gadrasil
Cervarix (HPV 16 and 18 and cross protection w/ hpv 31,33,35)
Gardasil (HPV 16 and 18 and cross protection w/ hpv 31,33,35) and ( HPV 6 and 11)
Gardasil-9: all cervarix and gardasil plus (HPV 31, 33, 45, 52, 58)
• ~40-45nm icosahedral, naked, circular double-stranded DNA
1
2
3
4
• Believed to be transmitted through respiratory or oral secretions
• Infections of these viruses usually occur during childhood and appears to have little clinical significance.
• Latency in __ and ___ can result symptomatic reactivation
• MOT: Respiratory droplets
Polyomavirus
- John Cunningham (JC), BK Virus, Merkel Cell Polyomavirus,Trichodysplasia spinulosa polyomavirus
- kidney and B lymphocytes
• Common infection of childhood
• Had been isolated in urine from children with ___ and evidence of
infection has been demonstrated in children with ___
• Reactivation in renal transplant patients- ____,
bone marrow transplant patients-___
• Treatment: ___
• Diagnosis:
1
2
BK Virus
- cystitis
- acute respiratory illness
- urethral structures
- hemorrhagic cystitis
- Cidofovir
- PCR (blood and urine), Electron Microscopy of urinary sediments
• Clinical importance is in patients who are immunosuppressed due to__ in whom the virus reactivates to cause ___
• Diagnosis: __,___,___
JC Virus
- T-cell dysfunction
- progressive multifocal leukoencephalopathy (PML)
- PCR (Brain biopsy or CSF), Serological Assays, Histological examinations
• Clinical importance is in patients who are immunosuppressed due to__ in whom the virus reactivates to cause ___
• Diagnosis: __,___,___
JC Virus
- T-cell dysfunction
- progressive multifocal leukoencephalopathy (PML)
- PCR (Brain biopsy or CSF), Serological Assays, Histological examinations
integrates its DNA into the genome of __
• Diagnosis: __, __
Treatment:__,___
Merkel Cell Polyomavirus
- Merkel Cells
- Tumor biopsy, PCR
- Surgery, Immunotherapy
• Growth of abnormal hair-like structures in the skin
• Clinical presentation: red scaly patches on skin, itching or discomfort, hair like growths
• Diagnosis: __
Treatment: __,__,__
Trichodysplasia Spinulosa Polyomavirus
- Skin biopsy |
- Cidofovir cream, Corticosteriods,
Laser therapy
