Hematocrit hema Flashcards
Volume of packed red blood cells that occupies a given volume of
whole blood after centrifugation of a blood sample
Also known as ____ or ____
Reported either as ___,___,___,___
Hematocrit
- Packed Cell Volume (PCV) or Erythrocyte Volume Fraction (EVF)
-percentage(%), cell volume percent (CV%), volume percent (Vol%) or in liters per liter (L/L)
Plasma
water, nutrients proteins hormones
Buffy coat
wbc, platelets
Hematocrit
RBC
Normal blood
37-47% hct female
42-52% hct male
depressed hct %
Anemia
elevated hct %
Polycythemia
HEMATOCRIT
DETERMINATION
Macro method ( Wintrobe method) & micro method (Adam’s Microhematocrit)
Adam’s Microhematocrit method
Procedure:
1. Fill two plain capillary tubes approximately three-quarters (3/4) full with blood anticoagulated with ___
> 2/3 filled, ____, filled portion ___
Length:___
Bore: ___
____ - for blood collected on anticoagulated tube
____ - for blood from skin puncture
- EDTA/heparin
-0.05 ml blood - 5 cm
- 7-7.5 cm
- 1.2 mm
- Non-heparinized (blue ring) capillary tube
- Heparinized (red ring) capillary tube
Seal the end of the tube with the colored ring using___
> Seal by placing the dry end into the tray with sealing compound at a __.
> Rotate the tube slightly and remove it from the tray
> The plug should be at least___ long
nonabsorbent sealing clay
90-degree angle
4 mm
Balance the tubes in a
microhematocrit centrifuge with the clay ends facing ___ , touching the rubber gasket
outside away from the center
Tighten the head cover on the
centrifuge and close the top. Centrifuge the tubes at ___ for ___.
10,000g to 15,000 g for 4-5 minutes
Determine the hematocrit by using a ___
microhematocrit reading device
The values of duplicate hematocrits
should agree within ___
1% (0.1 L/L)
HCT Reference Values
Conventional Unit:
Male: 40-54%
Female: 35-49%