LE Cell preparation Flashcards
is a neutrophil or macrophage that has phagocytised (engulfed) the denatured nuclear material of another cell. The denatured material is an ___
* Characteristic of___ but
also found in similar connective tissue disorders
LE cells
- absorbed haematoxylin body (basophilic particle also called an LE body)
- lupus erythematosus
Patients that have an abnormal plasma protein that causes swelling and breakdown of certain blood cell nuclei in vitro
This degenerated nuclear material attracts phagocytic cells, particularly segmented
__, which engulf this nuclear mass.
* The resulting phagocytes and inclusion material is termed as____
Systemic Lupus Erythematosus
- neutrophils
- “LE cell”
is a chronic, sometimes fatal disease of unknown etiology.
* ___ - peculiar skin eruption across nose and cheeks
>Often not present
>Diagnosis depends on the demonstration of LE cell
* __,__,__
Systemic Lupus Erythematosus
- Butterfly rash
- Leukopenia, thrombocytopenia, elevated sedimentation rate
___ are broken down in vitro allowing the abnormal plasma protein to react on the altered nuclear material
* Incubation enhances the __ and___
* Slides are prepared and examined for LE cells
Rotary bead method
- Leukocytes
- nuclear deterioration and phagocytises
Positive report should not be made without identification of this cell. The inclusion body with the leukocyte is ___ and ___. This feature distinguishes
the true LE cells from___
Rotary bead method
- homogenous
- no chromatin pattern
- tart cell (nucleophagocytosis)
contains engulfed, damaged
nucleus, usually that of ___, which still contains recognizable chromatin pattern
and distinct nuclear membrane
> __ with engulfed nuclear material
⚬ not to be equated with LE cell - no
diagnostic value
Tart cell
- lymphocyte
- monocyte/histiocyte
Procedure:
1. Prepare the ___ blood. Add ___
into the tube.
2.Rotate on a blood mixing rotor at ___ for __
3.Place the tube at ___ for ___
4.Transfer blood to a __,it at ___
5 Remove ____ and then remove the ___ carefully
8. Make smears of the ___ on clean and dry glass slides.
9. Fix the air dried smears in __ and stain with__
10. Examine under __
Rotary bead method
- heparinized
- 5 glass beads
- 50 rpm for 30 -minutes
- 37 degrees Celsius for 10 minutes
- Wintrobe tube
- 3000 rpm for 30 mins
- hemolyzed serum
- buffy coat
- buffy coat
- methanol
- Wright’s stain
- OIO
consideration
The ideal temperature to perform this test is __ and the process may be hastened by incubation
at ___
22 degrees celsius
37 degrees Celsius
LE cell test is considered positive when approximately ___ of the cells seen on the slide in the neutrophil count are LE cells.
2%-30%
Smear is considered positive when ___ characteristic LE cells are seen during a __
10 or more
15-min search
Negative findings on Le cell testing exclude diagnosis of ___
SLE
Le cell Positive also in
1
2
3
4
5
6
7
⚬ Rheumatoid arthritis
⚬ Chronic hepatitis (lupoid)
⚬ Scleroderma
⚬ Dermatomyositis
⚬ Polyarteritis nodosa
⚬ Acquired hemolytic anemia
⚬ Hodgkin disease
