P2 5d cloning Flashcards
1
Q
how do you micro propagate plants in vitro?
A
- cells are scraped from the parent plant cells
- the surface of those are sterilised then rinsed with water
- those are put into a sterile Petri dish containing sterile nutrient agar
- the medium encourage the cells to grow and divide making masses of cells
- each callus is put into a fresh growth medium that contains a range of plant growth regulators, causes them to develop roots, stems and leaves
- plantlet will develop into
2
Q
what are the advantages of micropropagation?
A
- clones are genetically identical
- important commercial uses
- allows plants desirable characteristics to be cloned
- ensures diseases in other areas are not imported and spread
- preserving rare plant species
- cheap
- big yield
- any time
- quick
3
Q
what are the disadvantages of micropropagation?
A
- trained personnel and sterile laboratory are required
- genetically identical makes them to be vulnerable to some diseases and pests
- no genetic variation makes them less adaptable
4
Q
how does embryo cloning work?
A
- egg cells are artificially fertilised using sperm cells taken from the best bull
- forms an embryo
- animal embryo is split apart many times before they become specialised
- cloned embryos are transplanted into host mothers
- offspring are born genetically identical
5
Q
how does adult cell cloning work?
A
- nucleus is removed from an unfertilised egg cell
- the nucleus from an adult body cell is inserted into the egg cell
- an electric shock stimulates the egg cell to divide (by mitosis) to form an embryo
- embryo cels contain same genetic information as the adult skin cell
- when the embryo is developed into a ball to cells, it is inserted into the womb of an adult female to continue development
6
Q
what are the benefits of cloning?
A
- be used to preserve endangered species of plants and animals
- allows farmers to ensure consistently high quality livestock
7
Q
what are the risks of cloning?
A
- reduced gene pool
- less resistance to disease
- cloned animals may not be as healthy
- worry for cloning of humans
- unethical
8
Q
what is pharming?
A
cloning animals that have a specific gene that is helpful to humans and create human proteins
when cloning it makes a a herd or flock