Oxidative Phosphorylation + Mitochondrial Function Flashcards
What connects the TCA cycle to the oxidative phosphorylation process?
a
How many protons are transported across the inner mitochondrial membrane upon oxidation of 1 NADH/FAD(2H)?
a
How many ATPs can be generated thru oxidation of 1 NADH? 1 FAD(2H)?
NADH = 2.5 ATPs
FAD(2H) = 1.5 ATPs
The electrochemical potential is called the proton motive force because it represents the potential energy driving protons to return to the more negatively charged (acidic/alkaline) matrix
alkaline
ATP synthase has a #__ base subunits of _____
12 + Carbons
How many proton(s) are needed to run the ATP synthase b 1 base position?
1
How many ATPs are generated thru a complete turn of the ATP synthase?
12 / 4 = 3 ATPs
Can oxidation of 1 NADH drive a complete turn of the ATP synthase?
No; only 5/6 turn
What is the energy efficiency of OXPHOS for complete oxidation of 1 mole of NADH or FAD(2H)?
NADH = 34%
FAD(2H) = 27%
Where does the bulk of the energy from OXPHOS go?
Dissipated as heat to keep the body warm when necessary
What are the co-factors involved in ETC?
FMN, FAD, CoQ
What are the metal elements involved in ETC?
Fe-S + Cu
What are some traits of CoQ?
- 10 isoprenoid units
- Hydrophobic tail
- Kept in the membrane space
What is one of the main reasons for anemia?
Iron deficiency
How does DNP achieve weight loss?
Drains proton gradient w/o generating ATP (uncoupling)
