Oxidative Phosphorylation + Mitochondrial Function Flashcards
What connects the TCA cycle to the oxidative phosphorylation process?
a
How many protons are transported across the inner mitochondrial membrane upon oxidation of 1 NADH/FAD(2H)?
a
How many ATPs can be generated thru oxidation of 1 NADH? 1 FAD(2H)?
NADH = 2.5 ATPs
FAD(2H) = 1.5 ATPs
The electrochemical potential is called the proton motive force because it represents the potential energy driving protons to return to the more negatively charged (acidic/alkaline) matrix
alkaline
ATP synthase has a #__ base subunits of _____
12 + Carbons
How many proton(s) are needed to run the ATP synthase b 1 base position?
1
How many ATPs are generated thru a complete turn of the ATP synthase?
12 / 4 = 3 ATPs
Can oxidation of 1 NADH drive a complete turn of the ATP synthase?
No; only 5/6 turn
What is the energy efficiency of OXPHOS for complete oxidation of 1 mole of NADH or FAD(2H)?
NADH = 34%
FAD(2H) = 27%
Where does the bulk of the energy from OXPHOS go?
Dissipated as heat to keep the body warm when necessary
What are the co-factors involved in ETC?
FMN, FAD, CoQ
What are the metal elements involved in ETC?
Fe-S + Cu
What are some traits of CoQ?
- 10 isoprenoid units
- Hydrophobic tail
- Kept in the membrane space
What is one of the main reasons for anemia?
Iron deficiency
How does DNP achieve weight loss?
Drains proton gradient w/o generating ATP (uncoupling)
What are the initial symptoms of ppl who take DNP for weight loss?
Fatigue, feels hot, and perhaps hungry
Shivering thermogenesis is due to repeated muscle contraction. Why does brown fat manifest a brown color?
They contain a large number of mitochondria
