oxidative phosphorylation Flashcards

1
Q

how does NADH from the cytoplasm get in through the membrane

A

malate aspartate shuttle

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2
Q

decribe the malate aspartate shuttle

A
  1. NADH from glycolysis is used to generate malate from oxaloacetate in cytosol
  2. Malate transporters transfer malate to mitochondrial matrix.
  3. Malate conversion to
    oxaloacetate in TCA cycle
    generates NADH in addition to the malate that arises from Fumarate
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3
Q

what does succinte dehydrogenase generate

A

fumarate and FADH2

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4
Q

what is phosphoryl transport potential of ATP measured by

A

free energy change

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5
Q

what is electron transfer potential of NADH and FADH2 measured by

A

redox potential

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6
Q

what is redox potential

A

The standard redox potential E’o of a (reduced) substance X is a measure for how readily X donates an electron (in comparison with H2)

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7
Q

what does a negative Eo mean

A

reduced form of X has a lower affinity for electrons than H2, a positive E’o means the opposite

negative means they will donate them

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8
Q

what will things with positive Eo tend to do

A

accept electrons - higher affinity for them than H2

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9
Q

what complex doesnt pump protons

A

II

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10
Q

where do electrons form NADH enter the resp chain

A

complex I

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11
Q

where do electrons from FADH2 enter the resp chain

A

complex II

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12
Q

what is complex II part of

A

the TCA cycle

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13
Q

what do electrons ultimately do

A

transferred onto O2 to form H2O

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14
Q

what are cytochromes

A

proteins that contain a haem group as a functional co factor

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15
Q

what does haem contain

A

a Fe(II) ion that can take up and release electrons

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16
Q

which ATP subunit protrudes into the matrix

A

F1

17
Q

which are the rotor subunits

A

c, y and E