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PMU Biochemistry > Oxidation of Fatty Acids > Flashcards

Oxidation of Fatty Acids Flashcards

1
Q

What is the main source of FAs for oxidation

A

.

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2
Q

In Plasma, how are LCFAs and SCFAs transported

A

/

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3
Q

In cells, what are FAs attached to

A

.

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4
Q

Why is it called B-oxidation

A

.

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5
Q

Describe the energy in B-oxidation

A

.

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6
Q

Before Oxidation, what needs to happen to the FFAs

A

active FA

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7
Q

How does the Acyl CoA penetrate the IMM, after it has been made from FFAs

A

.

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8
Q

Describe the 4 steps of B-oxidation.

A

.

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9
Q

How many turnovers of B-oxidation does a FA go through with nC atoms

A

.

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10
Q

How many molecules of Acyl CoA is made from B-oxidation of a FA with nC atoms

A

.

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11
Q

How is a FA activated - where it occurs, what bond is formed, what bonds are cleaved, what enzyme is used

A

.

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12
Q

Where is Acyl CoA synthetases present and what reaction does it catalyse

A

.

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13
Q

What is the function of the carnitine shuttle

A

.

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14
Q

Describe the process of carnitine shuttle

A 
Carnitine Palmitoyltransferase I 
Carnitine Palmitoyltransferase II
Carnitine Acyltransferase I
Carnitine Acyltransferase II
Transport protein Translocase
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15
Q

What inhibits Carnitine Palmitoyltransferase I on the OMM

A

.

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16
Q

What is the structure of carnitine and acyl carnitine

17
Q

Where does B-oxidation take place in the mitochondria

18
Q
  1. Describe the Dehydrogenation step of B-oxidation - enzyme, reducing eqs, ATP, structures
19
Q
  1. Describe the Hydration step of B-oxidation - enzyme, structures
20
Q
  1. Describe the Dehydrogenation step of B-oxidation - enzyme, reducing eqs, ATP, structures
21
Q
    1. Describe the Cleavage step of B-oxidation - enzyme, structures,
22
Q

Describe what coenzyme is present in Acyl CoA DH - relate this to ATP production

23
Q

how many isoenzymes does Acyl CoA DH have

24
Q

Describe how B-oxidation occurs with odd chain FAs

A
  • b-Oxidation proceeds regularly till obtaining Propionyl-CoA
  • It is converted into Succinyl-CoA (metabolite of
    Citric Acid Cycle)
  • Propionyl-CoA of odd-chain length FA are the only moiety of FA used in gluconeogenesis for glucose.
25
Q

Describe the oxidation of unsaturated FAs

26
Q

Where does B-oxidation of LCFAs (20-22) occur

27
Q

What is the difference between B-oxidation in peroxisomes and matrix

A
  • H202 formed

- no ATP produced

28
Q

What oxidising enzyme is present in B-oxidation in peroxisomes

29
Q

What is the process of B-oxidation in peroxisomes

A

shortened until C8 (Octanoyl CoA)

30
Q

What regulates B-oxidation

A

CPT I

Allosteric inhibitor

PMU Biochemistry (17 decks)

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