OSPE Strep pneumoniae-bacterial pneumonia

Question 1

Specimens

Answer 1

SPUTUM, blood

Question 2

Microscopy

Answer 2

 Gram’s stain – Gram +ve cocci in prs (both intra & extracellularly)
 Quellung reaction Capsule
 India ink prepn.– Capsule
pus cells

Question 3

Culture:

Answer 3

 Blood Agar - alpha hemolytic colonies (draughtman’s colonies)-due to autolysin prod which lyses its own cell wall
 Chocolate agar

Question 4

Specific tests:

Answer 4

 Oxidase –ve, Catalase –ve
 Inulin fermentation +ve
 Bile solubility +ve
 Optochin sensitive
 Pathogenic to mice (lethal)

Question 5

case

Answer 5

A 60-year-old man
-high-grade fever with chills and rigors for the previous two days.
- mild chest pain and productive cough.
-X-ray chest showed consolidation in the right lower lobe.
- Gram staining showed pus cells with Gram positive, lanceolate- shaped diplococci.
AST:sensitive to penicillin

Question 6

serology

Answer 6

counter-current immunoelectrophoresis,latex agglutination to demonstrate capsular antigens