OSPE Cytomegalovirus

Question 1

What are the Specimen to be collected:

Answer 1

Blood, Bronchoalveolar lavage

Question 2

High risk group for CMV infections:

Answer 2

Organ transplants, immunodeficient persons and in premature infants

Question 3

DIAGNOSTIC TESTS:

Answer 3

• Serological tests for confirmation,
• Isolation of viruses
  Tissue culture and Immunofluorescence staining- human fibroblast cultures

Question 4

Buffy Coat blood culture method using white blood cells

Answer 4

Cytomegalovirus-infected cells show characteristic abundant cytoplasm with central granular eosinophilic zone and eccentric, slightly concave nucleus (Wright-Giemsa staining)

Question 5

inclusion bodies in the infected tissue-

Answer 5

owl’s eye intranuclear inclusion bodies

Question 6

Serological tests:

Answer 6

CF- Complement fixation- Presumptive diagnosis
* IHA- Hemagglutination inhibition
* IF-Immunofluorescenceassayfordetectionofviral
antigens
* IgM, IgG antibodies against CMV– ELISA (very specific)