OSPE neonatal meningitis

Question 1

case

Answer 1

A 28-days-old
fever, irritability, poor feeding of 1 week duration and right-sided focal seizures on the day of presentation.
* An elder brother had developed symptomatic pharyngitis with fever, sore and congested throat 10 days prior to the neonate’s disease.
-bulging fontanel

Question 2

CSF FINDINGD

Answer 2

-Turbid
incresed P
decreased glucose
increased protein
leucocytosis

Question 3

Gram stain

Answer 3

Gram positive cocci in chains, pus cells ++

Question 4

Culture medium

Answer 4

Blood agar with beta hemolytic pinpoint colonies

Question 5

biochem test

Answer 5

Test 1: Bacitracin sensitivity test- Isolate is resistant
* Test 2- CAMP test. Isolate is positive ; Central streak – Staphylococcus aureus

• Principle of CAMP test : S. aureus sphingomyelinase and CAMP factor of S. agalactiae are responsible for the CAMP reaction. Sphingomyelinase first hydrolyzes sphingomyelin to ceramide, making erythrocytes susceptible to the lytic activity of factor CAMP.

Question 6

other test

Answer 6

• Latex test kit for bacterial pathogens causing meningitis: Group B streptococci is positive ( clumps ++)
• Lancefield typing Latex agglutination kit : Clumps seen for Group B streptococci