organisation - effect of temperature and pH on enzymes

Question 1

what happens to activity of enzyme as temperature is increased?

Answer 1

activity of enzyme increases (reaction gets faster)

Question 2

why does enzyme get faster as temperature increases?

Answer 2

substrate are moving faster so there are more collisions per second between the substrate and active site

Question 3

what is the optimum temperature of enzymes?

Answer 3

a certain temperature where enzymes are working at their fastest possible rate. at this point there is a maximum frequency of successful collisions between substrate and active site

Question 4

what is optimum temperature for human enzymes?

Answer 4

around 37

Question 5

what happens as we increase temperature of enzyme past optimum?

Answer 5

activity of enzymes rapidly decreases to zero

Question 6

what does activity of enzyme decrease after optimum temp?

Answer 6

enzyme molecules vibrate and the shape of the active site changes so substrate can no longer fit perfectly into active site. it has denatured. it can no longer catalyse

Question 7

how does pH affect enzymes?

Answer 7

if too acidic or too alkaline then active site denatures

Question 8

example of enzyme working in acidic conditions

Answer 8

protease enzyme in stomach

Question 9

example of enzyme working best in alkaline conditions

Answer 9

enzyme related from pancreas into small intestine eg lipase