organisation - effect of temperature and pH on enzymes Flashcards

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1
Q

what happens to activity of enzyme as temperature is increased?

A

activity of enzyme increases (reaction gets faster)

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2
Q

why does enzyme get faster as temperature increases?

A

substrate are moving faster so there are more collisions per second between the substrate and active site

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3
Q

what is the optimum temperature of enzymes?

A

a certain temperature where enzymes are working at their fastest possible rate. at this point there is a maximum frequency of successful collisions between substrate and active site

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4
Q

what is optimum temperature for human enzymes?

A

around 37

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5
Q

what happens as we increase temperature of enzyme past optimum?

A

activity of enzymes rapidly decreases to zero

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6
Q

why does activity of enzyme decrease after optimum temp?

A

enzyme molecules vibrate and the shape of the active site changes so substrate can no longer fit perfectly into active site. it has denatured. it can no longer catalyse

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7
Q

how does pH affect enzymes?

A

if too acidic or too alkaline then active site denatures

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8
Q

example of enzyme working in acidic conditions

A

protease enzyme in stomach

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9
Q

example of enzyme working best in alkaline conditions

A

enzyme related from pancreas into small intestine eg lipase

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