Non-Inflamm Diarrhea

Question 1

NID Char

Answer 1

Hypersecretion or decreased absorption involving SI. Abrupt onset, brief duration.

Question 2

E. Coli Char

Answer 2

4 strains, gram neg rod

Question 3

E. Coli Res

Answer 3

GI of humans and other animals

Question 4

E. Coli Diag

Answer 4

All lactose ferm (McConkey agar), all Indole pos, all but EHEC ferment Sorbitol (SMAC plate)

Question 5

ETEC Clin

Answer 5

Travelers Diarrhea with watery diarrhea, abd cramps, nausea, low grade fever. Electrolyte imbalance.

Question 6

ETEC VF

Answer 6

1. Heat Labile enterotoxin (LT) = ADP ribosylating toxin acts on adenylae cyclase increasing cAMP
2. Heat Stabile enterotoxin (ST) = acts on guanylate cyclase increasing cGMP

Question 7

EAEC Clin

Answer 7

Infantile Diarrhea

Question 8

EAEC VF

Answer 8

1. Aggregate adherence fimbrae results in stacked brick bacteria that shortens microvili therefore decreasing abs area
2. EAST 1 = ST like enterotoxin (increases cGMP)

Question 9

EPEC Clin

Answer 9

Infantile Diarrhea

Question 10

EPEC VF

Answer 10

1. Intimin - binds to GI surface
2. TIR Protein- Increases adhesion and provides signal path for rearrangement of actin = attaching effacing infection (formation of pedestal like structure therefore reducing abs area)

Question 11

EHEC/STEC Clin

Answer 11

Watery diarrhea followed by bloody diarrhea, sever abd cramps, HUS (hemolytic uremic syndrome) and hemorrhagic colitis possible

Question 12

EHEC/STEC VF

Answer 12

1. Intimin - intial binding to GI
2. TIR protein
3. Shiga toxin (verotoxin) - disrupts protein synth

Question 13

EHEC/STEC Res

Answer 13

poorly cooked ground beef

Question 14

EHEC/STEC Diag

Answer 14

Sorbitol nonferm

Question 15

EHEC/STEC RF

Answer 15

aggregated by anti-biotics

Question 16

Effacement/Pedestal formation

Answer 16

EPEC/EHEC - Intimin will tight bind and produce a type 3 pili that inserts to host and injects TIR protein which sets up signal transduction allowing rearrangement of host cell actin to pedestal (decreases villi).

Question 17

Vibrio spp. Clin

Answer 17

NID and Cholera

Question 18

Vibrio spp. Char

Answer 18

Comma shaped gram neg rod

Question 19

Vibrio spp. Res

Answer 19

Humans, fish and shellfish

Question 20

Vibrio spp. Trans

Answer 20

Fecal Oral

Question 21

Vibrio spp. RF

Answer 21

Contaminated water; improperly cooked seafood. Liver disease for V. Vulnificus

Question 22

Vibrio spp. Diag

Answer 22

Clinical; Culture in Thiosulfate Citrate Agar

Bile Salt Sucrose agar (cholerea = flat and yellow; vuln or para = small, blue green center)

Question 23

V. Cholerea Clin

Answer 23

Profuse watery diarrhea (14-20L per day; rice water) and loss of skin turgor

Question 24

V. Cholerea VF

Answer 24

1. Toxin Regulated Pilli (TcpA) for attach
2. Cholerea Enterotoxin (CTx/Choleragen) - LT like, increases cAMP
3. Hemaglutination protease - degradation of cell to cell adherence

Question 25

V. Parahaemolyticus Clin

Answer 25

Watery diarrhea w/o blood or mucus, cramps, nausea +/- vomit. Secrets as enterotoxin.

Question 26

V. Vulnificus Clin

Answer 26

Watery diarrhea w/o blood or mucus, cramps, nausea +/- vomit (+/- nec fasc w/ bullae)

Question 27

V. Vulnificus Comp/Seq

Answer 27

Primarily septicemia may occur in those with decreased or absent gastric acid and liver disease allowing extra-intestinal spread

Question 28

V. Vulnificus RF

Answer 28

Raw Oysters

Question 29

Listeria Monocytogenes Clin

Answer 29

Listeriosis = NID

Question 30

Listeria Monocytogenes Char

Answer 30

Faculatative Intracell gram pos rod, non spore

Question 31

Listeria Monocytogenes VF

Answer 31

Act A, listeriolysin, Internalins

Question 32

Listeria Monocytogenes Res

Answer 32

Humans and Livestock

Question 33

Listeria Monocytogenes Trans

Answer 33

Fecal Oral

Question 34

Listeria Monocytogenes RF

Answer 34

Consumption of unpasteurized dairy products, potted and deli meats

Question 35

Listeria Monocytogenes Diag

Answer 35

Culture at 4C