NAGE COPY Flashcards

1
Q

Give some details of the degenerate nature of the genetic code, include examples of the extremes in your answer

A

pg 27

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2
Q
  1. Describe, with the aid of diagrams, the processes involved in transcribing a eukaryotic gene.
A

pg 13

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3
Q

What is RNA Processing?

A

pg 15

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4
Q

Summarise the elongation step of translation

A

pg 30

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5
Q

1)Summarise what takes place in nucleic acid hybridisation

A

pg 38

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6
Q

Describe and explain the details of the initiation step of translation

A

pg 28

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7
Q

Describe the process for the separation of DNA fragments

A

pg 37

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8
Q

Describe the Anatomy of a Gene

A

pg 15

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9
Q

Describe the features of the signal sequences

A

pg 33

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10
Q

Describe what non-coding RNAs are and give the details of examples of such.

A

pg 20

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11
Q
  1. Describe what is meant by a “Transcription factor”
A

pg 12

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12
Q

Describe the action of Transfer RNA

A

pg 28

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13
Q

Explain the relationship between DNA and Genes, and describe the function of genes

A

pg 11

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14
Q

2)Describe and explain the details of the termination step of translation

A

pg 31

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15
Q
  1. Distinguish “sense” and “anti-sense” DNA template strands.
A

pg 15

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16
Q

1)Summarise the termination step of translation

A

pg 31

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17
Q

Give an Example of a type of mutation in splice sites featuring in ahuman disease

A

pg 19

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18
Q

Why is it referred to as a basal transcription complex

A

pg 13

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19
Q

Describe the Mechanism by which Aminoacyl tRNA Synthetases work

A

pg 28

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20
Q

Describe and explain the whole process of translation

A

pg 29-32

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21
Q

Other than adding dNTPs to the 3’ end what is the other role of DNA polymerase

A

pg 9

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22
Q

Give the details of what takes place in RNA interference

A

pg 23

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23
Q

Recall the Structure of a typical mRNA

A

pg 27

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24
Q

Explain why siRNAs are suitable to be used as therapies for genes we do not want to be expressed

A

pg 24

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25
Q

escribe an EXAMPLE of Post-Translational Modification - INSULIN

A

pg 36

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26
Q
  1. List the major functional classes of RNA and the classes of RNA polymerases involved in synthesising each of these.
A

pg 12

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27
Q

Describe the Post-transcriptional modification of the gene after splicing

A

pg 18

28
Q

Name examples of post-translational modification, and explain a quality that it increases

A

pg 36

29
Q

Describe the experimental evidence for the function of Micro RNAs

A

pg 25

30
Q

Describe how MRNA is read

A

pg 28

31
Q

Recall details of how problems with Micro RNAs can lead to Human Disease

A

pg 26

32
Q

Compare siRNAs with miRNAs

A

pg 21

33
Q

Explain examples of the uses of hybridisation(not sure of this part)

A

pg 39

34
Q
  1. Define “Transcription” and summarise the process including some detail on the type of proteins and/or sequences involved
A

pg 12

35
Q

Explain two methods through which transcription factors wor

A

pg 14

36
Q

Give examples of various hybridisation techniques and the type of nucleic acids they include

A

pg 39

37
Q

Summarise the details of the human Genome

A

pg 11

38
Q
  1. Describe what is meant by a “gene promoter”
A

pg 12

39
Q

Give EXAMPLES of Microarray Applications:

A

pg 42

40
Q

Describe Applications of PCR

A

pg 41

41
Q

How can miRNA be used in bioinformatics

A

pg 26

42
Q

Describe Gene Regulation in Human Disease

A

pg 14

43
Q

Describe the importance of DICER

A

pg 24

44
Q

Describe what a DNA (Oligonucleotide) Microarrays is

A

pg 41

45
Q

Summarise the initiation step of translation

A

pg 28

46
Q

2)Describe and explain the details of the elongation step of translation

A

pg 30

47
Q

Explain the C-value paradox

A
48
Q

Summarise the cell cycle

A

pg 9

49
Q

2) Describe the function of restriction endonucleases

A

pg 37

50
Q

Describe the process of Protein Synthesis on rER

A

pg 34

51
Q

Describe the Sequence of events in mRNA splicing

A

pg 16

52
Q

Describe how Antisense RNA can be used as a tool to block mRNA function

A

pg 23

53
Q

Give the details of miRNA Transcription and Processing

A

pg 25

54
Q

IN the context of other parts of nucleic acid analysis, describe what takes place in hybridisation

A

pg 38

55
Q

Recall Factors affecting Transcription Factor Expression

A

pg 14

56
Q

What is the relationship between the transciption complex and RNA polymerase II

A

pg 13

57
Q

Give examples of antibiotic and state their mechanisms

A

pg 33

58
Q

Give an exemplification of the Importance of Analysing Nucleic Acids

A

pg 37

59
Q

Exaplain Melting Temperature and Hybridisation Stringency, and the factors that affect it

A

pg 40

60
Q

Describe the PCR Primer Design

A

pg 41

61
Q

Explain the relationship between Antibiotics and Protein Synthesis

A

pg 33

62
Q
  1. Describe the basic differences between DNA and RNA
A

pg 11

63
Q

escribe how the process of Polymerase Chain Reaction is carried out

A

pg 41

64
Q

Describe Gene Expression in eukaryotic organisms

A

pg 11

65
Q

1)Describe the process of cell based DNA Cloning

A

pg 37

66
Q

Describe how efficiency is improved in translation

A

pg 33

67
Q

What is the main method used by transcription factors in their function

A

pg 13