Mycoviro special topic Flashcards
True or false
Swabs are generally not recommended for filamentous fungi
true
BSL For H. capsulatum, C. immitis, B. dermatitidis
BSL III
General rule transport at ? TEMPERATURE preferably within ? hours.
Room tempt; 2 hrs
Delays in non-sterile specimens collection store at
4C
Delays in normally sterile specimens collection store at
37C
True or false
Overnight specimens can be allowed for skin, hair, and nails
Cultures and specimen for mycology should be processed in a
Class
II BSC
urine is a normally sterile specimen
True or false
False
True or false
Normally sterile are specimens with organism
false
specimens should be sdisinfected with ?
70% alcohol
Direct exam
10% KOH
mount
Filamentousfungi rarely
grow on blood except Fusarium
true or false
true
Specimen used that needs to be Heparinized; for
Histoplasma;
bedside
inoculation
bone marrow
Direct exam for india ink for what specimen
CSF
preferred urine specimen with min volume
1st early
morning urine
(around 10 ml)
true or false
calcium alginate and swabs with wooden shafts can activate enveloped viruses
false; inactivate
true or false
For delays in processing, store specimens at 4C for no more than 5 days
* For loner delays and specimen transport, maintain at -70C for 6 or more
days
true
For Sin nombre virus, hepatitis, lassa fever,
rubella, arboviruses, HIV
preffered sample
Serum
Usually used for detection of viruses that infect
the lungs (CMV, influenza virus, adenovirus);
brain (HSV); GI tract (CMV)
Tissue specimens
– collect from the lower
conjunctiva using a flexible, fone shafted
dacron or rayon swab moistened with sterile
NSS and placed in VTM
Conjuctival swabs
SOC
Rhinovirus
nasal specimen
SOC
enterovirus
throat swab
rectal stool swab
SOC
HSV
throat swab
skin membrane lession
Sterile body fluids
Blood
SOC for CMv, BK Virus mumps, measles, adenovirus, norovirus,
urine
