BIOCHEM PART OF CC

Question 1

Folin Wu and Nelson-Somogyi is a ? reaction

Answer 1

Reduction Oxidation reaction

Question 2

Oxidation of Cuprous ion by

Answer 2

Phosphomolybdic acid

Question 3

Folin Wu product reaction
a. phosphomildbdenum
b. neocuproine
c. arsenomolybdenum

Answer 3

phosphomildbdenum

Question 4

Nelson-Somogyi product reaction
a. phosphomildbdenum
b. neocuproine
c. arsenomolybdenum

Answer 4

arsenomolybdenum

Question 5

Common initial reaction of the Copper Reduction.

Answer 5

Benedict’s reaction

Question 6

true or false
Phosphomildbdenum and Arsenomolybdenum is yellow orange in color. Cuprous-neocuproine complex is blue in color

Answer 6

FALSE BOTH
- Phosphomildbdenum and Arsenomolybdenum is blue in color
- Cuprous-neocuproine complex is yellow orange in color

Question 7

All are chemical method, except

1. Copper reduction, condensation, Glucose oxidase
2. Trinder reaction and condensation
3. Hexokinase and Glucose oxidase

a. 1 and 2
b. all of the above
c. none of the above
d. 2 and 3
e. 3 only

Answer 7

3 only
Chemical methods are
Copper reduction, condensation, Ferric reduction

Question 8

Most popular and most commonly used enzymatic method. Hydrogen peroxide is used as an oxidizing agent for this method

a. Glucose oxidase
b. Hexokinase
c. Glucose Dehydrogenase

Answer 8

Glucose oxidase

Question 9

Glucose Dehydrogenase is Measure using ?

a. NAD at A 340 nm
b. NADPH at A 360 nm
c. NADPH at A 340 nm
d. NAD at A 360 nm

Answer 9

NADPH at A 340 nm

Question 10

INBORN ERRORS Due to a deficiency of the enzyme galactose-1-phosphate uridyl
transferase

a. Hepatic glycogenosis
b. Muscle glycogenosis
c. Galactosemia
d. none of the above

Answer 10

Galactosemia

Question 11

Most common hepatic glycogenosis and is Deficient of the enzyme glucose-6-phosphatase

a. Muscle glycogenosis
b. von Gierke disease
c. hexokinase disease
d. Galactosemia

Answer 11

von Gierke disease

Question 12

true or false
von Gierke disease is Present with hypoglycemia, lactic acidosis, and hyperuricemia.

Answer 12

true

Question 13

CDC reference method; Involves saponification with alcoholic potassium hydroxide, extraction
with Bloor’s, and colorimetry using Liebermann

a. Modified Abell-Kendall
b. Salkowski
c. Liebermann-Burchardt

Answer 13

Modified Abell-Kendall

Question 14

Liebermann-Burchardt reaction product and color

a. cholestadienyl DSA; red
b. cholestadienyl MSA; red
c. cholestadienyl DSA; green
d. cholestadienyl MSA; green

Answer 14

cholestadienyl MSA; green

Question 15

Salkowski reaction product and color
a. cholestadienyl DSA; red
b. cholestadienyl MSA; red
c. cholestadienyl DSA; green
d. cholestadienyl MSA; green

Answer 15

a. cholestadienyl DSA; red

Question 16

Autosomal recessive disorder characterized by low to undetectable HDL due
to a mutation in the ABCA1 gene
* Defect in HDL
A. Hypoalphalipoproteinemia
B. Betalipoprotenimia
C. Tangier disease

Answer 16

Tangier disease

Question 17

Tangier disease* Defect in HDL
* Common autosomal dominant disorder characterized by HDL-C Levels
→ <30 mg/dL in men
→ <40 mg/dL in women
* High risk for CHD because of low HDL level
* HDL is inversely related to cardiac risk.
A. Hypoalphalipoproteinemia
B. Betalipoprotenimia
C. Tangier disease

Answer 17

Hypoalphalipoproteinemia

Question 18

the only step that is modified in the
original Abell-Kendall.

A. Saponification
B. Extraction
C. Purification
D. Colorimetry

Answer 18

Extraction