Mycoplasma

Question 1

Cell wall–free bacteria

Answer 1

Mycoplasma

Question 2

Are the smallest organisms that can be free living in nature and self-replicating on
laboratory media.

Answer 2

Mycoplasmas

Question 3

May live Intracellularly as well as extracellularly.

Answer 3

Mycoplasmas

Question 4

Causes pneumonia and has been associated with joint and other infections. Normal flora of the oropharynx of fewer than 5% of adult

Answer 4

Mycoplasma pneumoniae

Question 5

Sometimes causes postpartum fever and has been found with other bacteria in uterine tube infections (Salpingitis), Pyelonephritis (10 %), tubo-ovarian abscesses

Answer 5

Mycoplasma hominis

Question 6

Is a cause of nongonococcal urethritis in men and is associated with lung
disease in premature infants of low birth weight.

Answer 6

Ureaplasma urealyticum

Question 7

Is closely related to M pneumoniae and has been associated with urethral
and other urogenital infections, can also cause non-gonococcal urethritis, cervicitis, endometritis, salpingitis, and infertility

Answer 7

Mycoplasma genitalium

Question 8

Is a prominent cause of pneumonia, especially in persons 5–20 years
of age.

Answer 8

Mycoplasma pneumoniae

Question 9

Clinical presentation Mycoplasma pneumoniae

Answer 9

• mild pneumoniae to serious pneumonitis,
• with occasional neurologic and hematologic (ie, hemolytic anemia) involvement
• a variety of possible skin lesions.
• Can also cause Bullous myringitis (Inflammation of the TM)

Question 10

Mycoplasma are highly pleomorphic because they lack a rigid cell wall and
instead are bounded by a _______ that contains a sterol (mycoplasmas require the addition of serum or cholesterol to the medium to produce sterols for growth)

Answer 10

triple-layered “unit membrane”

Question 11

Mycoplasmas are completely resistant to _______ because they lack the
cell wall structures at which penicillin acts, but they are inhibited by _________

Answer 11

• penicillin

* tetracycline or erythromycin

Question 12

Mycoplasmas can reproduce in cell-free media; _______, the center of the
whole colony is characteristically embedded beneath the surface

Answer 12

on agar

Question 13

Growth on this consists principally of protoplasmic masses of indefinite shape that are easily distorted.

Answer 13

solid media

Question 14

A metabolic substrate such as glucose or urea, and growth factors such as yeast extract that is required in culture of mycoplasmas that cause disease in humans

Answer 14

media with

serum

Question 15

Incubation of cultured mycoplasma

Answer 15

After incubation at 37°C for 48–96 hours

Question 16

Are a complex group of interactive proteins in ycoplasma

Answer 16

Adhesins

Question 17

Adhesins of M pneumoniae

Answer 17

P1 adhesin

Question 18

Adhesins of M genitalium

Answer 18

MgPa adhesin

Question 19

Influence the protein folding and binding and are important in the adherence to cells.

Answer 19

Adherence Accessory Proteins

Question 20

Treatment: of M pneumoniae

Answer 20

Tetracyclines and erythromycins- DOC

Question 21

Treatment: of M genitalium urethritis in men

Answer 21

a single dose of azithromycin administered in the clinic

Question 22

Direct examination of a specimen for mycoplasmas

Answer 22

useless

Question 23

Agar media in mycoplasma is best incubated at

Answer 23

37°C with 5–10% CO2 (under microaerophilic conditions or even anaerobic conditions

Question 24

Colonies of M hominis may have a typical appearance on agar

Answer 24

“fried egg” appearance

Question 25

Specimens submitted for diagnosis of Ureaplasma species are usually inoculated to _______. Growth is signaled by a color change indicating hydrolysis of urea

Answer 25

broth or agar media (eg, A8 agar) containing urea

Question 26

Glycolipid antigens extracted with chloroform methanol from cultured mycoplasmas

Answer 26

Complement Fixation

Question 27

Using group O human erythrocytes appear in about 50% of untreated patients, in rising titer, with the maximum reached in the third or fourth week after onset. A titer of 1:64 or more supports the diagnosis of M pneumoniae infection

Answer 27

Cold hemagglutinins detection for M. pneumoniae